Soft shell of pistachio (Pistacia vera L.) pyrolysis experiments were performed in a fixed-bed reactor to produce bio-oil. The effects of temperature, heating rate, and sweep gas (N-2) flow rates on the yields and compositions of products were investigated. Pyrolysis runs were performed using reactor temperatures between 350 and 500 degrees C with heating rates of 15 and 50 degrees C/min. Nitrogen flow rates varied between 50 and 200 cm(3)/min and mean particle size was 0.8 mm. The maximum bio-oil yield of 33.18% was obtained in a nitrogen atmosphere with nitrogen flow rate of 150 cm(3)/min and at 450 degrees C pyrolysis temperature with a heating rate of 50 degrees C/min.The elemental analysis and gross heating value of the bio-oil were determined, and then the chemical composition of the bio-oil was investigated using chromatographic and spectroscopic techniques. The chemical characterization has shown that the bio-oil obtained from soft shell of pistachio can be used as a renewable fuel and chemical feedstock.