We report on the fluorescence enhancement induced by silver island film (SIF) deposited on a silicon wafer. The model immunoassay was studied on silvered and on silvered wafers. The fluorescence brightness of Rhodamine Red-X increased about 300% on the SIF, while the lifetime was reduced by several fold and the photostability increased substantially. We discuss potential uses of silicon wafer substrates in multiplex assays in which the fluorescence is enhanced due to the SIF, and the multiplexing is achieved by using micro-transponders. Published by Elsevier B.V.