Enhanced Adsorption of Protein Fused with Polymeric Material-binding Peptide

Matsuno H; Date T; Kubo Y; Yoshino Y; Tanaka N; Sogabe A; Kuroita T; Serizawa T

Chemistry Letters, Vol.38, No.8, 834-835, 2009

DOI10.1246/cl.2009.834 Export Citation

Enhanced Adsorption of Protein Fused with Polymeric Material-binding Peptide

Matsuno H, Date T, Kubo Y, Yoshino Y, Tanaka N, Sogabe A, Kuroita T, Serizawa T

A model protein was fused with the ELWRPTR peptide, which binds specifically to the isotactic poly(methyl methacrylate) (it-PMMA) film surface, and its adsorption on PMMA film surfaces was then investigated by surface plasmon resonance measurements. We found that the adsorption constant of the fusion protein for the it-PMMA film surface was 75-fold greater than that of native protein.