A new cuprous adsorbent was prepared by bioreduction method with biomass R08 and its desulfurization ability and regenerative property at low temperature were studied. The results indicated that the amount of SO2 adsorbed on the adsorbent was lower than that on Cu/gamma-Al2O3 prepared by chemical method at low temperature; however, it can be easily regenerated and the regenerative adsorbent still has a good SO2 adsorption ability, higher than 87% of that in fresh adsorbent after six regenerations. XPS and FTIR analysis showed that biomass can reduce Cu2+ to Cu+ by aldose and keep the valence of copper exposed to O-2 and H2O atmosphere. Furthermore, SO2 is adsorbed on the sorbent with chemical complexation bonds so that it can be desorbed by increasing temperature which is different from gamma-Al2O3, Cu(II)/gamma-Al2O3 and Cu(I)/gamma-Al2O3 that sulfate species are formed.