Identification of mutation sites on Delta 12 desaturase genes from Mortierella alpina 1S-4 mutants

Sakuradani E; Abe T; Matsumura K; Tomi A; Shimizu S

Journal of Bioscience and Bioengineering, Vol.107, No.2, 99-101, 2009

DOI10.1016/j.jbiosc.2008.10.011 Export Citation

Identification of mutation sites on Delta 12 desaturase genes from Mortierella alpina 1S-4 mutants

Sakuradani E, Abe T, Matsumura K, Tomi A, Shimizu S

The mutation sites on the Delta 12 desaturase gene in Mortierella alpino Delta 12 desaturase-defective mutants SR88, TM912, and Mut48 accumulating Mead acid were identified. Each mutation resulted in an amino acid replacement (H116Y and P166L) in the Delta 12 desaturase gene from SR88 and Mut48, respectively. (C) 2008, The Society for Biotechnology, Japan. All rights reserved.

Keywords:Mortierella alpine;Delta 12 desaturase;Mutant;Mead acid;Polyunsaturated fatty acid