Screening, purification, and identification of the enzyme producing N-(L-alpha-L-aspartyl)-L-phenylalanine methyl ester from L-isoasparagine and L-phenylalanine methyl ester

Kira I; Asano Y; Yokozeki K

Journal of Bioscience and Bioengineering, Vol.108, No.3, 190-193, 2009

DOI10.1016/j.jbiosc.2009.03.018 Export Citation

Screening, purification, and identification of the enzyme producing N-(L-alpha-L-aspartyl)-L-phenylalanine methyl ester from L-isoasparagine and L-phenylalanine methyl ester

Kira I, Asano Y, Yokozeki K

Screening was carried out for microorganisms able to produce N-(L-alpha-L-aspartyl)-L-phenylalanine methyl ester [APM] from L-isoasparagine and L-phenylalanine methyl ester hydrochloride. Of the 422 strains examined, 44 strains belonging to the family Enterobacteriaceae were found to produce APM. The enzyme catalyzing APM production was purified and identified as dipeptidaseE. (C) 2009, The Society for Biotechnology, Japan. All rights reserved.

Keywords:N-(L-alpha-aspartyl)-L-phenylalanine-1-methyl ester;L-isoasparagine;L-phenylalanine methyl ester;Dipeptidase E;Screening