Journal of Bioscience and Bioengineering, Vol.108, No.4, 354-356, 2009
In vivo circular RNA production using a constitutive promoter for high-level expression
The permuted intron-exon (PIE) method based on group I intron self-splicing is the only methodology currently available for production of circular RNA in vivo. Here, we report improvement of the circular RNA expression method based on an induction-free vector system utilizing the highly efficient constitutive lpp promoter. (C) 2009, The Society for Biotechnology, Japan. All rights reserved.