BACKGROUND: A recent innovation in fixed film bioreactors is the pulsed plate bioreactor (PPBR) with immobilized cells. The successful development of a theoretical model for this reactor relies on the knowledge of several parameters, which may vary with the process conditions. It may also be a time-consuming and costly task because of their nonlinear nature. Artificial neural networks (ANN) offer the potential of a generic approach to the modeling of nonlinear systems. RESULTS: A feedforward ANN based model for the prediction of steady state percentage degradation of phenol in a PPBR by immobilized cells of Nocardia hydrocarbonoxydans (NCIM 2386) during continuous biodegradation has been developed to correlate the steady state percentage degradation with the flow rate, influent phenol concentration and vibrational velocity (amplitude x frequency). The model used two hidden layers and 53 parameters (weights and biases). The network model was then compared with a Multiple Regression Analysis (MRA) model, derived from the same training data. Further these two models were used to predict the percentage degradation of phenol for blind test data. CONCLUSIONS: The performance of the ANN model was superior to that of the MRA model and was found to be an efficient data-driven tool to predict the performance of a PPBR for phenol biodegradation. (C) 2008 Society of Chemical Industry.