Poly(N-isopropylacrylamide-co-dimethylamino ethyl methacrylate)가 고정화된 알지네이트 비드 제조 및 방출 특성

강미경; 김진철; Mi Kyoung Kang; Jin-Chul Kim

Polymer(Korea), Vol.34, No.1, 79-83, January, 2010

Preparation and Release Property of Alginate Beads Immobilizing Poly(N-isopropylacrylamide-co-dimethylamino ethyl methacrylate)

강미경, 김진철 ^†

강원대학교 생명공학부 생물소재공학전공

Mi Kyoung Kang, and Jin-Chul Kim^†

School of Biotechnology & Bioengineering and Institute of Bioscience and Biotechnology, Kangwon National University, 192-1, Hyoja 2 dong, Chunchon, Gangwon-do 200-701, Korea

E-mail:

초록

Poly(N-isopropylacrylamide-co-dimethylamino ethyl methacrylate)(P(NIPAM-co-DMAEMA))가 고 정화된 알지네이트 비드를 제조하였다. 알지네이트와 P(NIPAM-co-DMAEMA)의 정전기적 인력을 이용하여 P(NIPAM-co-DMAEMA)로 코팅된 알지네이트 비드와 P(NIPAM-co-DMAEMA)을 함유한 알지네이트 비드를 제조하였다. P(NIPAM-co-DMAEMA)은 자유 라디칼 반응으로 제조하였고 핵자기 공명분광기를 통해 확인하였다. 비드의 온도 민감성 방출 거동을 관찰하였으며, 모델시약으로 blue dextran과 fluorescein isothiocyanate-dextran을 사용하였다. P(NIPAM-co-DMAEMA)가 고정화된 알지네이트 비드로부터의 방출 정도는 온도 의존성이 낮았고, 방출모델시약의 분자량이 작을수록 더 높았다.

Alginate beads were prepared using poly(N-isopropylacrylamide-co-dimethylamino ethyl methacrylate)(P(NIPAM-co-DMAEMA)). First, P(NIPAM-co-DMAEMA) was immobilized on the surface of alginate beads by taking advantage of electrostatic interaction between alginate and P(NIPAM-co-DMAEMA). Second, P(NIPAM-co-DMAEMA) was contained in the matrix of alginate beads. P(NIPAM-co-DMAEMA) were prepared by a free radical polymerization at 74 ℃ for 12 h. The weight ratio of NIPAM to DMAEMA monomer was 95/5. The copolymer was identified by 1H-NMR. Releases from the alginate beads were observed at 30, 37, and 45 ℃ using blue dextran or FITCdextran(fluorescein isothiocyanate-dextran) as a model drug. The effect of temperature on the degree of release from the beads was insignificant. FITC-dextran was released more than blue dextran possibly due to its smaller molecular weight.

Keywords:alginate beads;N-isopropylacrylamide;dimethylamino ethyl methacrylate;release property

[References]

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Cheng CJ, Chu LY, Zhang J, Zhou MY, Xie R, Desalination, 234(1-3), 184 (2008)
Yamashita K, Nishimura T, Nango M, Polym. Adv. Technol., 14, 189 (2003)
Duracher D, ElaoEssari A, Pichot C, Colloid Polym. Sci., 277, 905 (1999)
Zhang H, Chu LY, Li YK, Lee YM, Polymer, 48(6), 1718 (2007)
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Kono K, Henmi A, Yamashita H, Hayashi H, Takagishi T, J. Control. Rel., 59, 63 (1999)
Choi JH, Lee HY, Kim JC, Kim YC, J. Ind. Eng. Chem., 13(3), 380 (2007)
Hua JD, Liu YF, Hu J, Wang QQ, Gong ZB, Guo XZ, J. Appl. Polym. Sci., 74(10), 2457 (1999)
Choi JH, Lee HY, Kim JC, J. Appl. Polym. Sci., 108(6), 3707 (2008)
Zhang J, Xie R, Zhang SB, Cheng CJ, Ju XJ, Chu LY, Polymer, 50, 2516 (2009)
Feil H, Bae YH, Feijen J, Kim SW, Macromolecules, 26, 2496 (1993)
van Tomme SR, van Steenbergen MJ, de Smedt SC, van Nostrum CF, Hennink WE, Biomaterials, 276, 2129 (2005)
Yang X, Lee HY, Kim JC, J. Macromol. Sci. Part A-Pure Appl. Chem., 46, 959 (2009)
Karlsson D, Zacchi G, Axelsson A, Biotechnol. Prog., 18(6), 1423 (2002)
Ende MT, Peppas NA, J. Control. Rel., 48, 47 (1997)

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[3] Ertesvag H, Valla S, Polym. Degrad. Stabil., 59, 85 (1998)

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[10] Yamashita K, Nishimura T, Nango M, Polym. Adv. Technol., 14, 189 (2003)

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[18] Kono K, Henmi A, Yamashita H, Hayashi H, Takagishi T, J. Control. Rel., 59, 63 (1999)

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[20] Hua JD, Liu YF, Hu J, Wang QQ, Gong ZB, Guo XZ, J. Appl. Polym. Sci., 74(10), 2457 (1999)

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[22] Zhang J, Xie R, Zhang SB, Cheng CJ, Ju XJ, Chu LY, Polymer, 50, 2516 (2009)

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[24] van Tomme SR, van Steenbergen MJ, de Smedt SC, van Nostrum CF, Hennink WE, Biomaterials, 276, 2129 (2005)

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