Raman microspectroscopy (RM), temperature-programmed oxidation (TPO), high-resolution transmission electron microscopy (HRTEM), and electron energy loss spectroscopy (EELS) were combined to get comprehensive information oil the relationship between structure and reactivity of soot in samples of spark discharge (GfG), heavy duty engine diesel (EURO VI and IV) soot, and graphite powder upon oxidation by oxygen at increasing temperatures. GfG soot and graphite powder represent the higher and lower reactivity limits. Raman microspectroscopic analysis was conducted by determination of spectral parameters using a five band fitting, procedure (G, D1-D4) as well as by evaluation of the dispersive character of the D mode. The analysis of spectral parameters shows,I higher degree of disorder and a higher amount Of Molecular carbon for Untreated GfG soot samples than for samples Of untreated EURO VI and EURO IV soot. The Structural analysis based oil the dispersive Character of the D mode revealed substantial differences in ordering descending from graphite powder, EURO IV, VI to GfG soot. HRTEM Images and EELS analysis of EURO IV and VI samples indicated it different morphology and a higher Structural order its compared to GfG soot in full agreement with the Raman analysis. These findings are also confirmed by the reactivity of soot during oxidation (TPO), where GfG soot was Found to be the most reactive and EURO IV and VI soot samples exhibited I moderate reactivity.