The supercritical fluid extraction of grape seed (Vitis vinifera L) oil using carbon dioxide has been carried out at constant temperature (313.15 K) and solvent flow rate (1.7 x 10(-4) kg s(-1)), at 160, 180 and 200 bar, using both untreated and enzymatically pre-treated seeds. The pre-treatment of triturated seeds has been performed with a cell wall degrading enzyme cocktail containing cellulase, protease, xylanase, and pectinase, in order to enlarge the broken/intact cells ratio, thus increasing oil availability. The maximum extraction yield obtained was 16.5%, which is 44% higher than the 11.5% yield obtained with untreated seeds. The cumulative extraction curves measured show two characteristic periods: a first linear part where the majority of the oil is obtained, and a second asymptotic branch which contributes with only 3-8% to the total oil removed. As pressure rises, the mass Of CO2 needed to reach a definite extraction yield decreases and the linear part of the extraction curves of treated and untreated seed approach themselves. (C) 2008 Elsevier B.V. All rights reserved.