In the telomere region, T-loop structure is thought to participate in protecting chromosome ends. Although the tetraplex structure, called G-quadruplex, is suggested to form in the same region, the relationship between these two structures are less discussed. Here we designed the long lariat formation using G-quadruplex structure. The structural property was characterized by means of CD spectroscopy, FRET experiment, and native PAGE analysis. Because this lariat formation contains both loop structure and tetraplex structure, it can be a key structure linking T-loop structure to G-quadrurplex structure.