Cell patterning on a poly(N-vinyl pyrrolidone)-patterned polystyrene substrate by using ion implantation

Mi-Young Ahn; In-Tae Hwang; Chan-Hee Jung; Young-Chang Nho; Jae-Hak Choi; Kang Moo Huh

Journal of Industrial and Engineering Chemistry, Vol.16, No.1, 87-90, January, 2010

DOI10.1016/j.jiec.2010.01.015 Export Citation

Cell patterning on a poly(N-vinyl pyrrolidone)-patterned polystyrene substrate by using ion implantation

Mi-Young Ahn^{1, 2}, In-Tae Hwang¹, Chan-Hee Jung¹, Young-Chang Nho¹, Jae-Hak Choi^{1, †}, and Kang Moo Huh²

¹Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, 1266 Sinjeong-dong, Jeollabuk-do 580-185, Republic of Korea
²Department of Polymer Science and Engineering, Chungnam National University, Yuseong-gu, Daejeon 305-701, Republic of Korea

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In this study, a simple strategy for micropatterning of cells was developed by using ion implantation that does not require any harsh chemicals and complicated processes. Thin poly(vinyl pyrrolidone) (PVP) films spin-coated on a nonbiological polystyrene Petri dishes were implanted with accelerated proton ions through a pattern mask and then developed with water to generate the patterns of the PVP. The results of the ATR-FTIR and XPS analysis revealed that the chemical compositions of the PVP were not significantly changed by ion implantation and thus the intrinsic biocompatibility of the PVP can be preserved. The in vitro cell culture on the patterned PVP showed selective alignment of cells on the PVP regions of the patterns and thus well-defined 100 μm patterns of the cells were obtained. These results revealed that this strategy is biocompatible and simple to use for biomolecular patterning, which can be used in further biological applications.

Keywords:Poly(N-vinyl pyrrolidone);Micropatterning;Ion implantation;Cell culture

[References]

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Falconnet D, Csucs G, Grandin HM, Textor M, Biomaterials, 27, 3044 (2006)
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Suh KY, Seong JH, Khademhosseini A, Laibinis PE, Langer R, Biomaterials, 25, 557 (2004)
Kwon HJ, Jung CH, Hwang IT, Nho YC, Choi JH, J. Ind. Eng. Chem., 15(5), 703 (2009)
Bosetti M, Masse A, Tobin E, Cannas M, J. Mater. Sci.: Mater. Med., 12, 431 (2001)
Jagielski J, Piatkowska A, Aubert P, Thome L, Turos A, Kader AA, Surf. Coat. Technol., 200, 6355 (2006)
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Hong Y, Chirila TV, Vijayasekaran S, Shen W, Lou X, Dalton PD, J. Biomed. Mater. Res., 39, 650 (1998)
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Burkert S, Schmidt T, Gohs U, Monch I, Arndt KF, J. Appl. Polym. Sci., 106(1), 534 (2007)
An JC, J. Ind. Eng. Chem., 15(2), 148 (2009)
Encyclopedia of Chemistry website: http://www.chemie.de/lexikon/e/Poly_vinyl_pyrrolidone/
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He W, Halberstadt CR, Gonsalves KE, Biomaterials, 25, 2055 (2004)
Ganesan R, Yoo SY, Choi JH, Lee SY, Kim JB, J. Mater. Chem., 18, 703 (2008)
Can HK, Radiat. Phys. Chem., 72, 703 (2005)
Moharram MA, Khafagi MG, J. Appl. Polym. Sci., 105(4), 1888 (2007)
Chan K, Kostun LE, Tenhaeff WE, Gleason KK, Polymer, 47(20), 6941 (2006)
Sionkowska A, Wisniewski M, Kaczmarek H, Skopinska J, Chevallier P, Mantovani D, Lazare S, Tokarev V, Appl. Surf. Sci., 253(4), 1970 (2006)

[Related Articles]

[1] Kane RS, Takayama S, Ostuni E, Ingber DE, Whitesides GM, Biomaterials, 20, 2362 (1999)

[2] Falconnet D, Csucs G, Grandin HM, Textor M, Biomaterials, 27, 3044 (2006)

[3] Malkov GS, Martin IT, Schwisow WB, Chandler JP, Wickes BT, Gamble LJ, Castner DG, Fisher ER, Plasma Process. Polym., 5, 129 (2008)

[4] Favia P, Sardella E, Gristina R, d’Agostino R, Surf. Coat. Technol., 169, 707 (2003)

[5] Suh KY, Seong JH, Khademhosseini A, Laibinis PE, Langer R, Biomaterials, 25, 557 (2004)

[6] Kwon HJ, Jung CH, Hwang IT, Nho YC, Choi JH, J. Ind. Eng. Chem., 15(5), 703 (2009)

[7] Bosetti M, Masse A, Tobin E, Cannas M, J. Mater. Sci.: Mater. Med., 12, 431 (2001)

[8] Jagielski J, Piatkowska A, Aubert P, Thome L, Turos A, Kader AA, Surf. Coat. Technol., 200, 6355 (2006)

[9] Kwon HJ, Jung CH, Kim DK, Lim YM, Kim HK, Nho YC, Choi JH, J. Korean Phys. Soc., 52, 819 (2008)

[10] Jung CH, Kim DK, Hwang IT, Lim YM, Kim HK, Nho YC, Choi JH, Nucl. Instrum. Meth. B, 267, 1089 (2009)

[11] Hong Y, Chirila TV, Vijayasekaran S, Shen W, Lou X, Dalton PD, J. Biomed. Mater. Res., 39, 650 (1998)

[12] Sionkowska A, Eur. Polym. J., 39, 2135 (2003)

[13] Sen M, Avcı EN, J. Biomed. Mater. Res. A, 74, 187 (2005)

[14] Burkert S, Schmidt T, Gohs U, Monch I, Arndt KF, J. Appl. Polym. Sci., 106(1), 534 (2007)

[15] An JC, J. Ind. Eng. Chem., 15(2), 148 (2009)

[16] Encyclopedia of Chemistry website: http://www.chemie.de/lexikon/e/Poly_vinyl_pyrrolidone/

[17] He W, Gonsalves KE, Pickett JH, Halberstadt C, Biomacromolecules, 4(1), 75 (2003)

[18] He W, Halberstadt CR, Gonsalves KE, Biomaterials, 25, 2055 (2004)

[19] Ganesan R, Yoo SY, Choi JH, Lee SY, Kim JB, J. Mater. Chem., 18, 703 (2008)

[20] Can HK, Radiat. Phys. Chem., 72, 703 (2005)

[21] Moharram MA, Khafagi MG, J. Appl. Polym. Sci., 105(4), 1888 (2007)

[22] Chan K, Kostun LE, Tenhaeff WE, Gleason KK, Polymer, 47(20), 6941 (2006)

[23] Sionkowska A, Wisniewski M, Kaczmarek H, Skopinska J, Chevallier P, Mantovani D, Lazare S, Tokarev V, Appl. Surf. Sci., 253(4), 1970 (2006)