Removal of neurotoxic ethyl parathion pesticide by two-stage chemical/enzymatic treatment system using Fenton’s reagent and organophosphorous hydrolase

Suk Soon Choi; Sang Hwan Seo; Dong Gyun Kang; Jeong Hyub Ha; Hyung Joon Cha

Korean Journal of Chemical Engineering, Vol.27, No.3, 900-904, March, 2010

DOI10.1007/s11814-010-0115-9 Export Citation

Removal of neurotoxic ethyl parathion pesticide by two-stage chemical/enzymatic treatment system using Fenton’s reagent and organophosphorous hydrolase

Suk Soon Choi^†, Sang Hwan Seo, Dong Gyun Kang¹, Jeong Hyub Ha², and Hyung Joon Cha^{1, †}

Department of Biological and Environmental Engineering, Semyung University, Jecheon 390-711, Korea
¹National Research Laboratory of Molecular Biotechnology, Department of Chemical Engineering, Pohang University of Science and Technology, Pohnag 790-784, Korea
²Department of Environmental Science, Kangwon National University, Chuncheon 200-701, Korea

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Organophosphate compounds, which are essential ingredients of pesticide, plasticizer, air fuel, and chemical warfare agents, are serious neurotoxic hazardous materials. Therefore, diverse physical-chemical treatments have been attempted to degrade organophosphate compounds. In the present work, we propose a two-stage chemical and enzymatic treatment system. As a first stage, pretreatment of oxidation and coagulation using Fenton’s reagent utilizing iron and hydrogen peroxide was employed. Preferentially, 1 mM ethyl parathion (EP) pesticide was largely (~80%) removed by Fenton’s reagent reaction within 15 min. To remove residual EP, enzymatic treatment with organophosphorous hydrolase (OPH) from recombinant Escherichia coli was employed as a second stage. We successfully demonstrated that the proposed two-stage hybrid treatment process removed 1mM environmental toxic EP efficiently (~98%) within 30 min.

Keywords:Organophosphate Compound;Ethyl Parathion Pesticide;Two-stage Treatment;Fenton’s Reagent;Organophosphorous Hydrolase

[References]

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Hoppin JA, Umbach DM, London SJ, Alavanja MCR, Sandler DP, Am. J. Respir. Crit. Care Med., 165, 683 (2002)
Richins RD, Mulchandani A, Chen W, Biotechnol. Bioeng., 69(6), 591 (2000)
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Yu JJ, Water Res., 36, 1095 (2002)
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Huston PL, Pignatello JJ, Water Res., 33, 1238 (1999)
Ku Y, Lin HS, Water Res., 36, 4155 (2002)
Badawy MI, Ghaly MY, Gad-Allah TA, Desalination, 194(1-3), 166 (2006)
Doong RA, Chang WH, Chemosphere, 37, 2563 (1998)
Diagne M, Oturan N, Oturan MA, Chemosphere, 66, 841 (2007)
Chen S, Sun DZ, Chung JS, J. Hazard. Mater., 144(1-2), 577 (2007)
Hong SH, Kwon BH, Lee JK, Kim IK, Korean J. Chem. Eng., 25(1), 46 (2008)
Lin CY, Biol. Wastes, 34, 215 (1990)
McDaniel CS, Harper LL, Wild JR, J. Bacteriol., 170, 2306 (1998)
Mulbry WW, Karns JS, J. Bacteriol., 171, 6740 (1998)
Kang DG, Choi SS, Cha HJ, Biotechnol. Prog., 22(2), 406 (2006)
Kang DG, Kim JYH, Cha HJ, Biotechnol. Lett., 24(11), 879 (2002)
Li L, Kang DG, Cha HJ, Biotechnol. Bioeng., 85(2), 214 (2004)
Kang DG, Lim GB, Cha HJ, J. Biotechnol., 118, 339 (2005)
Wu CF, Cha HJ, Valdes JJ, Bentley WE, Biotechnol. Bioeng., 77(2), 212 (2002)
Caldwell SR, Raushel FM, Biotechmol. Bioeng., 37, 103 (1991)
Chamarro E, Marco A, Esplugas S, Water Res., 35, 1047 (2001)

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[1] Singh BK, Walker A, Fems Microbiol. Rev., 30, 428 (2006)

[2] Hoppin JA, Umbach DM, London SJ, Alavanja MCR, Sandler DP, Am. J. Respir. Crit. Care Med., 165, 683 (2002)

[3] Richins RD, Mulchandani A, Chen W, Biotechnol. Bioeng., 69(6), 591 (2000)

[4] Guivarch E, Oturan N, Oturan MA, Environ. Chem. Lett., 1, 165 (2003)

[5] Yu JJ, Water Res., 36, 1095 (2002)

[6] Chiron S, Fernandez-Alba A, Rodriguez A, Garcia-Calvo E, Water Res., 34, 366 (2000)

[7] Huston PL, Pignatello JJ, Water Res., 33, 1238 (1999)

[8] Ku Y, Lin HS, Water Res., 36, 4155 (2002)

[9] Badawy MI, Ghaly MY, Gad-Allah TA, Desalination, 194(1-3), 166 (2006)

[10] Doong RA, Chang WH, Chemosphere, 37, 2563 (1998)

[11] Diagne M, Oturan N, Oturan MA, Chemosphere, 66, 841 (2007)

[12] Chen S, Sun DZ, Chung JS, J. Hazard. Mater., 144(1-2), 577 (2007)

[13] Hong SH, Kwon BH, Lee JK, Kim IK, Korean J. Chem. Eng., 25(1), 46 (2008)

[14] Lin CY, Biol. Wastes, 34, 215 (1990)

[15] McDaniel CS, Harper LL, Wild JR, J. Bacteriol., 170, 2306 (1998)

[16] Mulbry WW, Karns JS, J. Bacteriol., 171, 6740 (1998)

[17] Kang DG, Choi SS, Cha HJ, Biotechnol. Prog., 22(2), 406 (2006)

[18] Kang DG, Kim JYH, Cha HJ, Biotechnol. Lett., 24(11), 879 (2002)

[19] Li L, Kang DG, Cha HJ, Biotechnol. Bioeng., 85(2), 214 (2004)

[20] Kang DG, Lim GB, Cha HJ, J. Biotechnol., 118, 339 (2005)

[21] Wu CF, Cha HJ, Valdes JJ, Bentley WE, Biotechnol. Bioeng., 77(2), 212 (2002)

[22] Caldwell SR, Raushel FM, Biotechmol. Bioeng., 37, 103 (1991)

[23] Chamarro E, Marco A, Esplugas S, Water Res., 35, 1047 (2001)