Horizontally and vertically aligned helical polyacetylenes (H-PAs) are synthesized by using orientation-control led chiral nematic liquid crystals (N*-LCs) consisting of vertical orientation inducers, chiral dopants and parent N-LC. The chemically graspable Pictures of horizontal and vertical N*-LCs and the corresponding macroscopically aligned H-PAs are put forward as a guideline of the syntheses of the N*-LCs and H-PAs. It is found that the present N*-LCs show horizontal and vertical orientations, although they include the vertical orientation inducer. Systematic examinations indicate that the horizontal or vertical orientation of the N*-LC sensitively depends on (i) the relative concentrations of the vertical orientation inducer and the chiral dopant, (ii) the miscibility of the vertical orientation inducer to the parent N-LC, and (iii) the helical twisting power of the chiral dopant. In other words, the balance in effective strength between the vertical orientation inducer and the chiral dopant dominates the macroscopic orientation of the N*-LC and hence the morphological alignment of the H-PA.