The electrochemical oxidation of glucose in 1M NaOH at Pt/PVC-bonded RuO2 composite electrode, fabricated using RuO2 powders prepared at 300 degrees C, occurs via surface redox mediation by Ru(VI)/Ru(VII) intermediates, following Michaelis-Menten kinetics. The related kinetic parameters are evaluated from Lineweaver-Burke plots. The improved response and the extended detection limit for glucose estimation are analysed.