In this study, the influence of plant hormones, negatively charged indolilo-3-acetic acid (IAA) and positively charged zeatin, on lipid membranes was studied. As models of negatively and positively charged biological membranes, mononolayers of 1,2-dimyristoyl-sn-glycero-3-[phospho-L-serine] (DMPS) and 1,2-dipalmitoyl-3-trimethylammoniumpropane (DPTAP) at the water/air interface were used, respectively, Additionally, the effect of cadmium and selenium ions on the interactions between hormones and lipids was Studied. Surface pressure and Surface potential measurements, Brewster angle microscopy (BAM), and grazing incidence X-ray diffraction (GIXD) were used for that purpose. Both IAA and zeatin led to ail expansion of the lipid monolayer caused by electrostatic interactions between oppositely charged groups: negatively charged polar group of DMPS and positively charged zeatin or positive DPTAP headgroup and negative IAA. The addition of ions to the subphase containing hormones causes competitive interactions of both Solutes with oppositely charged lipid polar heads. The largest effect was observed for IAA. While Zeatin does not change the domain shape of DMPS, IAA causes the complete disappearance of characteristic DPTAP domains, Addition of SCO42- ions causes restoration of DPTAP domains observed on pure water.