히알루론산이 함유된 SIS 스폰지와 섬유륜세포를 이용한 디스크재생

홍희경; 이선경; 송이슬; 김대성; 엄신; 김형은; 이동원; 강길선; Hee Kyung Hong; Seon Kyoung Lee; Yi-Seul Song; Dae Sung Kim; Shin Eom; Hyoung Eun Kim; Dongwon Lee; Gilson Khang

Polymer(Korea), Vol.34, No.3, 282-288, May, 2010

Export Citation

히알루론산이 함유된 SIS 스폰지와 섬유륜세포를 이용한 디스크재생

Biodisc Regeneration Using Annulus Fibrosus Cell with Hyaluronic Acid Impregnated Small Intestinal Submucosa Sponge

홍희경, 이선경, 송이슬, 김대성, 엄신, 김형은, 이동원 ^†, 강길선 ^†

전북대학교 BIN 융합공학과, 고분자나노공학과

Hee Kyung Hong, Seon Kyoung Lee, Yi-Seul Song, Dae Sung Kim, Shin Eom, Hyoung Eun Kim, Dongwon Lee^†, and Gilson Khang^†

Department of BIN Fusion Technology & Department of Polymer·Nano Science & Technology, Chonbuk National University, 664-14 Dukjin Ku, Jeonju 561-756, Korea

E-mail:,

초록

소장점막하조직(SIS)은 면역반응이 없어 생체물질로 널리 사용되고 있다. 히알루론산(HA)은 천연 보습제라 불릴 정도로 친수성이 매우 뛰어나고 조직을 수복하기 위한 체내이식용 구조물로써 널리 사용되고 있는 천연고분자이다. 본 연구에서는 섬유륜세포를 이용한 조직공학 디스크 재생을 위해 HA를 함유한 SIS/HA 스폰지를 제조하였다. 제조된 스폰지 내부의 전자현미경 관찰 결과 열린 다공구조를 하고 있음을 확인하였고, FTIR 분석을 통해 C-O 관능기가 관찰되어 SIS/HA 스폰지 내부의 HA가 빠져나가지 않고 고르게 분포되어 있음을 확인하였다. 결론적으로 SIS/HA 스폰지는 SIS와 HA 각각의 특성을 유지하며 섬유륜세포의 성장에 유익한 환경을 제공함을 확인하였고, 이는 HA의 우수한 생체적합성 및 수분 보유능력에 기인된 것으로 사료된다.

The porcine small intestinal submucosa (SIS) has been widely used as a biomaterial without immuno rejection responses and hyalunonic acid (HA) can be used as biocompatible materials to regenerate tissue. We developed the SIS sponge and HA loaded SIS sponges (SIS/HA) for the possibility of the application of the tissue engineering using annulus fibrosus (AF). SEM observation shows that SIS and SIS/HA sponges have interconnected and open pores. We demonstrated the presence of HA in SIS/HA sponge from C-O functional group observed by the FTIR analysis. In conclusion, we suggest that SIS/HA sponge may be useful to tissue engineering using AF cell. This may be due to the enhanced biocompatibility and higher water retention capacity of HA.

Keywords:small intestinal submucosa;hyaluronic acid;sponge;annulus fibrosus

[References]

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Bibby SR, Jones DA, Lee RB, Yu J, Urban JP, Joint Bone Spine, 68, 537 (2001)
Lim JY, Kim SH, Park SW, So JW, Back MO, Kim MS, Khang G, Rhee JM, Lee HB, Tissue Eng. Regen. Med., 5, 96 (2008)
Hedbom E, Heinegard D, J Biol. Chem., 264, 6898 (1989)
Hedbom E, Heinegard D, J Biol. Chem., 268, 27307 (1993)
Cassinelli EH, Hall RA, Kang JD, Spine J., 1, 205 (2001)
Gruber HE, Leslie K, Ingram J, Norton HJ, Hanley EN, Spine J., 4, 44 (2004)
Ko YK, Kim SH, Ha HJ, Kim MS, Han CW, Rhee JM, Son YS, Lee HB, Khang G, Tissue Eng. Regen. Med., 4, 67 (2007)
Maldonado BA, Oegema TR, J. Orthop. Res., 10, 677 (1992)
Chiba K, Andersson GB, Masuda K, Spine J., 22, 2885 (1997)
Gruber HE, Fisher EC, Desai JB, Stasky AA, Hoelscher G, Hanley EN, Exp. Cell Res., 235, 13 (1997)
Shin HW, Kim SH, Jang JW, Kim MS, Cho SH, Lee HB, Khang G, Polym. Sci. Tech., 2, 194 (2004)
Bdylak SF, Kropp B, McPherson TB, Tissue Eng., 4, 379 (1998)
Ledet EH, Carl AL, Dirisio DJ, Tymeson MP, Andersen LB, Sheenhanm CE, Kallakury B, Slivka M, Serhan H, Spine J., 2, 188 (2002)
Prestwich G, Marecak D, Marecek J, Vercruysse K, Ziebell M, J. Control. Release, 53, 93 (1998)
Park SN, Lee HJ, Lee KH, Seo H, Biomaterials, 24, 1631 (2003)
Thalhammer JG, Vladimirova M, Bershadsky B, Anesthesiology, 82, 1013 (1995)
Lim JY, Kim SH, Khang G, Rhee JM, Polym.(Korea), 32(5), 415 (2008)
Park KS, Jin CM, Yun SJ, Hong KD, Kim SH, Kim MS, Rhee JM, Khang G, Rhee JM, Lee HB, Tissue Eng. Regen. Med., 5, 96 (2008)
Forato LA, Filho RB, Colnago A, Anal. Biochem., 15, 136 (1998)
Lee EK, Lee JS, Park HS, Kim CH, Gin YJ, Son YS, Tissue Eng. Regen. Med., 2, 94 (2005)
Khang G, Lee SJ, Han CW, Rhee JM, Lee HB, Adv. Exp. Med. Biol., 534, 235 (2003)
Lee K, Roper JG, Wang J, Tissue Eng. Regen. Med., 5, 437 (2008)
Ha HJ, Kim SH, Yoon SJ, Ko YK, Lee EK, Son Y, Kim MS, Rhee JM, Khang G, Lee HB, Tissue Eng. Regen. Med., 3, 416 (2006)
Ko YK, Kim SH, Jeong JS, Ha HJ, Yoon SJ, Rhee JM, Kim MS, Lee HB, Khang G, Polym.(Korea), 31(1), 14 (2007)
Choi BS, Kim SH, Yun SJ, Ha HJ, Kim MS, Yang YI, Son Y, Khang G, Rhee JM, Lee HB, Tissue Eng. Regen. Med., 3, 295 (2006)
Entwistle J, Hall CL, Turley EA, J. Cell Biochem., 61, 569 (1996)

[Referenced By]

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[1] Buckwalter JA, Spine J., 20, 1307 (1995)

[2] Bibby SR, Jones DA, Lee RB, Yu J, Urban JP, Joint Bone Spine, 68, 537 (2001)

[3] Lim JY, Kim SH, Park SW, So JW, Back MO, Kim MS, Khang G, Rhee JM, Lee HB, Tissue Eng. Regen. Med., 5, 96 (2008)

[4] Hedbom E, Heinegard D, J Biol. Chem., 264, 6898 (1989)

[5] Hedbom E, Heinegard D, J Biol. Chem., 268, 27307 (1993)

[6] Cassinelli EH, Hall RA, Kang JD, Spine J., 1, 205 (2001)

[7] Gruber HE, Leslie K, Ingram J, Norton HJ, Hanley EN, Spine J., 4, 44 (2004)

[8] Ko YK, Kim SH, Ha HJ, Kim MS, Han CW, Rhee JM, Son YS, Lee HB, Khang G, Tissue Eng. Regen. Med., 4, 67 (2007)

[9] Maldonado BA, Oegema TR, J. Orthop. Res., 10, 677 (1992)

[10] Chiba K, Andersson GB, Masuda K, Spine J., 22, 2885 (1997)

[11] Gruber HE, Fisher EC, Desai JB, Stasky AA, Hoelscher G, Hanley EN, Exp. Cell Res., 235, 13 (1997)

[12] Shin HW, Kim SH, Jang JW, Kim MS, Cho SH, Lee HB, Khang G, Polym. Sci. Tech., 2, 194 (2004)

[13] Bdylak SF, Kropp B, McPherson TB, Tissue Eng., 4, 379 (1998)

[14] Ledet EH, Carl AL, Dirisio DJ, Tymeson MP, Andersen LB, Sheenhanm CE, Kallakury B, Slivka M, Serhan H, Spine J., 2, 188 (2002)

[15] Prestwich G, Marecak D, Marecek J, Vercruysse K, Ziebell M, J. Control. Release, 53, 93 (1998)

[16] Park SN, Lee HJ, Lee KH, Seo H, Biomaterials, 24, 1631 (2003)

[17] Thalhammer JG, Vladimirova M, Bershadsky B, Anesthesiology, 82, 1013 (1995)

[18] Lim JY, Kim SH, Khang G, Rhee JM, Polym.(Korea), 32(5), 415 (2008)

[19] Park KS, Jin CM, Yun SJ, Hong KD, Kim SH, Kim MS, Rhee JM, Khang G, Rhee JM, Lee HB, Tissue Eng. Regen. Med., 5, 96 (2008)

[20] Forato LA, Filho RB, Colnago A, Anal. Biochem., 15, 136 (1998)

[21] Lee EK, Lee JS, Park HS, Kim CH, Gin YJ, Son YS, Tissue Eng. Regen. Med., 2, 94 (2005)

[22] Khang G, Lee SJ, Han CW, Rhee JM, Lee HB, Adv. Exp. Med. Biol., 534, 235 (2003)

[23] Lee K, Roper JG, Wang J, Tissue Eng. Regen. Med., 5, 437 (2008)

[24] Ha HJ, Kim SH, Yoon SJ, Ko YK, Lee EK, Son Y, Kim MS, Rhee JM, Khang G, Lee HB, Tissue Eng. Regen. Med., 3, 416 (2006)

[25] Ko YK, Kim SH, Jeong JS, Ha HJ, Yoon SJ, Rhee JM, Kim MS, Lee HB, Khang G, Polym.(Korea), 31(1), 14 (2007)

[26] Choi BS, Kim SH, Yun SJ, Ha HJ, Kim MS, Yang YI, Son Y, Khang G, Rhee JM, Lee HB, Tissue Eng. Regen. Med., 3, 295 (2006)

[27] Entwistle J, Hall CL, Turley EA, J. Cell Biochem., 61, 569 (1996)