Preparation of Thermosensitive Gelatin-Pluronic Copolymer for Cartilage Tissue Engineering

Dong Hwa Kim; Su-Jin Heo; Jung-Woog Shin; Chi Woong Mun; Kyung Min Park; Ki Dong Park; Kyoung Soo

Macromolecular Research, Vol.18, No.4, 387-391, April, 2010

DOI10.1007/s13233-010-0405-4 Export Citation

Preparation of Thermosensitive Gelatin-Pluronic Copolymer for Cartilage Tissue Engineering

Dong Hwa Kim^{1, 2}, Su-Jin Heo^{1, 2}, Jung-Woog Shin^{1, 2}, Chi Woong Mun^{1, 2}, Kyung Min Park³, Ki Dong Park³, and Kyoung Soo^{4, †}

¹Team of BK21, Department of Biomedical Engineering, Inje University, Gyeongnam, 621-749, Korea
²FIRST Research Group, Institute of Biomedical Engineering, Inje University, Gyeongnam, 621-749, Korea
³Department of Molecular Science and Technology, Ajou University, Suwon, 443-749, Korea
⁴Department of Oral Biology & Institute of Oral Biology, School of Dentistry, Kyung Hee University, Seoul, 130-701, Korea

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In this study, a gelatin conjugated Pluronic® thermosensitive polymer(Gelatin-Pluronic, GP) was synthesized by the direct coupling of gelatin to monocarboxylated Pluronic®(MP) and characterized. The use of thermosensitive polymers has recently been proposed for many applications including tissue engineering, gene therapy, novel drug delivery systems, implantable devices, such as scaffolds for cartilage regeneration and nanotechnology. Water soluble thermosensitive GP was developed as an injectable scaffold using EDC/NHS as a coupling reagent for catilage regeneration. 1H NMR, and FTIR were used to characterize the chemical structure of the modified gelatins. The GP solution showed a reversible sol-gel transition behavior around body temperature. The cell viability was evaluated using methylthiazolyldiphenyl tetrazolium bromide (MTT) assay. The optical density (O.D.) of GP was significantly higher than that of Pluronic® at 1, 3 and 5 days, indicating that the cell viability on GP was better than on Pluronic®. Therefore, the GP copolymer can be used as an injectable cell-containing scaffold owing to its thermally reversible properties and good biocompatibility.

Keywords:gelatin;Pluronic®;thermosensitive hydrogel;cartilage;chondrocytes

[References]

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LaPrade RF, J. Bone. Joint Surg. Am., 85A, 2259 (2003)
Ghazavi MT, Pritzker KP, Davis AM, Gross AE, J. Bone Joint Surg. Br., 79, 1008 (1997)
Park KM, Bae JW, Joung YK, Shin JW, Park KD, Colloids Surf. B: Biointerfaces, 63, 1 (2008)
Jun YJ, Park KM, Joung YK, Park KD, Lee SJ, Macromol. Res., 16(8), 704 (2008)
Campoccia D, Doherty P, Radice M, Brun P, Abatangelo G, Williams DF, Biomaterials, 19, 2101 (1998)
Kabanov A, Self Assembly Appl., 347 (2000)
Malmstem M, Self Assembly Appl., 319 (2000)
Park KM, Lee SY, Joung YK, Na JS, Lee MC, Park KD, Acta Biomaterialia, 5, 1956 (2009)
Park KM, Joung YK, Park KD, Lee SY, Lee MC, Macromol. Res., 16(6), 517 (2008)
Liu Y, Chen F, Liu W, Cui L, Shang Q, Xia W, Tissue Eng., 8, 709 (2002)
Stern S, Lindenhayn K, Schultz O, Perka C, Acta Orthop. Scand., 71, 496 (2000)
Frost SJ, Weigel PH, Biochim. Biophys. Acta, 1034, 39 (1990)
Ray M, Hatcher S, Whitehouse SL, Crawford S, Crawford R, J. Thromb. Haemost., 3, 1421 (2005)
Ray MJ, Hales M, Marsh N, Blood Coagul. Fibrinolysis, 12, 129 (2001)
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Jabbur NS, J. Cataract Refract. Surg., 29, 1636 (2003)
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Eyrich D, Brandl F, Appel B, Wiese H, Maier G, Wenzel M, Staudenmaier R, Goepferich A, Blunk T, Biomaterials, 28, 55 (2007)
Park SH, Park SR, Min BH, Key Eng. Mater., 253, 342 (2007)
Heywood HK, Bader DL, Lee DA, J. Cell. Physiol., 206, 402 (2006)
Carrier RL, Papadaki M, Rupnick M, Schoen FJ, Bursac N, Langer R, Freed LE, Vunjak-Novakovic G, Biotechnol. Bioeng., 64(5), 580 (1999)
Park KM, Kim DH, Bae JW, Joung YK, Shin JW, Park KD, Biomaterials Research, 12, 24 (2008)
Mattii L, Battolla B, D’Alessandro D, Trombi L, Pacini S, Cascone MG, Lazzeri L, Bernardini N, Dolfi A, Galimberti S, Petrini M, Macromol. Biosci., 8, 819 (2008)
Huang Y, Onyeri S, Siewe M, Moshfeghian A, Madihally SV, Biomaterials, 26, 7616 (2005)
Xia W, Liu W, Cui L, Liu Y, Zhong W, Liu D, Wu J, Chua K, Cao Y, J. Biomed. Mater. Res. B: Appl. Biomater., 71, 373 (2004)
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[Referenced By]

[1] Buckwalter JA, Mankin HJ, Instr. Course Lect., 47, 477 (1998)

[2] LaPrade RF, J. Bone. Joint Surg. Am., 85A, 2259 (2003)

[3] Ghazavi MT, Pritzker KP, Davis AM, Gross AE, J. Bone Joint Surg. Br., 79, 1008 (1997)

[4] Park KM, Bae JW, Joung YK, Shin JW, Park KD, Colloids Surf. B: Biointerfaces, 63, 1 (2008)

[5] Jun YJ, Park KM, Joung YK, Park KD, Lee SJ, Macromol. Res., 16(8), 704 (2008)

[6] Campoccia D, Doherty P, Radice M, Brun P, Abatangelo G, Williams DF, Biomaterials, 19, 2101 (1998)

[7] Kabanov A, Self Assembly Appl., 347 (2000)

[8] Malmstem M, Self Assembly Appl., 319 (2000)

[9] Park KM, Lee SY, Joung YK, Na JS, Lee MC, Park KD, Acta Biomaterialia, 5, 1956 (2009)

[10] Park KM, Joung YK, Park KD, Lee SY, Lee MC, Macromol. Res., 16(6), 517 (2008)

[11] Liu Y, Chen F, Liu W, Cui L, Shang Q, Xia W, Tissue Eng., 8, 709 (2002)

[12] Stern S, Lindenhayn K, Schultz O, Perka C, Acta Orthop. Scand., 71, 496 (2000)

[13] Frost SJ, Weigel PH, Biochim. Biophys. Acta, 1034, 39 (1990)

[14] Ray M, Hatcher S, Whitehouse SL, Crawford S, Crawford R, J. Thromb. Haemost., 3, 1421 (2005)

[15] Ray MJ, Hales M, Marsh N, Blood Coagul. Fibrinolysis, 12, 129 (2001)

[16] van Oeveren W, Jansen NJ, Bidstrup BP, Royston D, Westaby S, Neuhof H, Wildevuur CR, Ann. Thorac. Surg., 44, 640 (1987)

[17] Jabbur NS, J. Cataract Refract. Surg., 29, 1636 (2003)

[18] Mol A, van Lieshout MI, Dam-de Veen CG, Neuenschwander S, Hoerstrup SP, Baaijens FP, Bouten CV, Biomaterials, 26, 3113 (2005)

[19] Eyrich D, Brandl F, Appel B, Wiese H, Maier G, Wenzel M, Staudenmaier R, Goepferich A, Blunk T, Biomaterials, 28, 55 (2007)

[20] Park SH, Park SR, Min BH, Key Eng. Mater., 253, 342 (2007)

[21] Heywood HK, Bader DL, Lee DA, J. Cell. Physiol., 206, 402 (2006)

[22] Carrier RL, Papadaki M, Rupnick M, Schoen FJ, Bursac N, Langer R, Freed LE, Vunjak-Novakovic G, Biotechnol. Bioeng., 64(5), 580 (1999)

[23] Park KM, Kim DH, Bae JW, Joung YK, Shin JW, Park KD, Biomaterials Research, 12, 24 (2008)

[24] Mattii L, Battolla B, D’Alessandro D, Trombi L, Pacini S, Cascone MG, Lazzeri L, Bernardini N, Dolfi A, Galimberti S, Petrini M, Macromol. Biosci., 8, 819 (2008)

[25] Huang Y, Onyeri S, Siewe M, Moshfeghian A, Madihally SV, Biomaterials, 26, 7616 (2005)

[26] Xia W, Liu W, Cui L, Liu Y, Zhong W, Liu D, Wu J, Chua K, Cao Y, J. Biomed. Mater. Res. B: Appl. Biomater., 71, 373 (2004)

[27] Lien SM, Ko LY, Huang TJ, Acta Biomater., 5, 670 (2009)