Testing red blood cell deformability of laboratory animals by slit-flow ektacytometry in various viscosity media : Inter-species and gender differences

Ferenc Kiss; Erika Sajtos; Lili Matyas; Zsuzsanna Magyar; Istvan Furka; Iren Miko; Norbert Nemeth

Korea-Australia Rheology Journal, Vol.22, No.2, 113-118, June, 2010

Ferenc Kiss, Erika Sajtos, Lili Matyas, Zsuzsanna Magyar, Istvan Furka, Iren Miko, and Norbert Nemeth^†

Department of Operative Techniques and Surgical Research, Institute of Surgery,Medical and Health Science Center, University of Debrecen, Hungary

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Viscosity of the suspending medium can be a determinant contributor to the effective measurements of red blood cell deformability using ektacytometry. Deformability of erythrocyte is known to show inter-species and gender differences, therefore the evaluation of the effects and the standardized usage of the suspending media are important in animal experiments. For evaluating the differences using various viscosity media, we aimed to test male and female Sprague-Dawley rats and beagle dogs. Blood samples were collected and red blood cell deformability was tested by a RheoScan-D200 slit-flow ektacytometer using parallel measurements in PVP solutions at viscosity of 15, 20 and 30 mPa.s. The lowest elongation index values were measured in 15 mPa.s solution in both species and genders. The inter-species differences were obvious: rats had higher elongation index values. Gender differences were found only in 20 and 30 mPa.s samples: in rats males, while in dogs females had better red blood cell deformability. The standard usage of PVP solutions is essential for comparative studies, because the results obtained from different viscosity PVP solutions are not comparable to each other. In Sprague-Dawley rats and beagle dogs the relatively less viscous media (<20 mPa.s) may result in less informative data.

Keywords:red blood cell deformability;inter-species differences;gender differences;ektacytometry;suspending medium viscosity

[References]

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Baskurt OK, Boynard M, Cokelet GC, Connes P, Cooke BM, Forconi S, Liao F, Hardeman MR, Jung F, Meiselman HJ, Nash G, Nemeth N, Neu B, Sandhagen B, Shin S, Thurston G, Wautier JL, Clin. Hemorheol. Microcirc., 42, 75 (2009)
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Bitbol M, Biophys. J, 49, 1055 (1986)
Fisher TM, Biophys. J., 93, 2553 (2007)
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ICSH Expert Panel on Blood Rheology, Clin. Hemorheol., 6, 439 (1986)
Meiselman HJ, Scand. J. Clin. Lab. Invest., 41, 27 (1981)
Musielak M, Clin. Hemorheol. Microcirc., 42, 47 (2009)
Nash GB, Meiselman HJ, Ann. N. Y. Acad. Sci., 416, 255 (1983)
Nemeth N, Alexy T, Furka A, Baskurt OK, Meiselman HJ, Furka I, Miko I, Biorheology, 46, 155 (2009)
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Pfafferott C, Nash GB, Meiselman HJ, Biophys. J., 47, 695 (1985)
Schmid-Schonbein H, Wells R, Science, 165, 288 (1969)
Shin S, Ku Y, Park MS, Suh JS, Cytometry Part B (Clinical Cytometry), 65, 6 (2005)
Stoltz JF, Singh M, Riha P, eds, Microrheological parameters, in: Hemorheology in Practice, IOS Press, Amsterdam, The Netherlands, pp. 27-52. (1999)
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[1] Armstrong JK, Wenby RB, Meiselman HJ, Fisher TC, Biophys. J., 87, 4259 (2004)

[2] Aydogan S, Yapislar H, Artis S, Aydogan B, Clin. Hemorheol. Microcirc., 39, 93 (2008)

[3] Baskurt OK, Boynard M, Cokelet GC, Connes P, Cooke BM, Forconi S, Liao F, Hardeman MR, Jung F, Meiselman HJ, Nash G, Nemeth N, Neu B, Sandhagen B, Shin S, Thurston G, Wautier JL, Clin. Hemorheol. Microcirc., 42, 75 (2009)

[4] Baskurt OK, Hardeman MR, Uyuklu M, Ulker P, Cengiz M, Nemeth N, Shin S, Alexy T, Meiselman HJ, Biorheology, 46, 251 (2009)

[5] Baskurt OK, Hardeman MR, Uyuklu M, Ulker P, Cengiz M, Nemeth N, Shin S, Alexy T, Meiselman HJ, Scan. J. Clin. Lab. Inv., 69, 777 (2009)

[6] Baskurt OK, Meiselman HJ, Clin. Hemorheol. Microcirc., 31, 23 (2004)

[7] Bitbol M, Biophys. J, 49, 1055 (1986)

[8] Fisher TM, Biophys. J., 93, 2553 (2007)

[9] Fisher T, Schmid-Schonbein H, Tank tread motion of red cell membranes in viscometric flow, in: Red Cell Rheology, Bessis M, Shohet SB, Mohandas N, eds, Springer Verlag, Berlin, pp. 347-358. (1978)

[10] Fritz Jr. OG, Biophys. J., 46, 219 (1984)

[11] Hardeman MR, Personal communication.

[12] Hardeman MR, Goedhart PT, Shin S, Methods in hemorheology, in: Handbook of Hemorheology and Hemodynamics, Baskurt OK, Hardeman MR, Rampling MW, Meiselman HJ, eds, IOS Press, Amsterdam, The Netherlands, pp. 242-266. (2007)

[13] ICSH Expert Panel on Blood Rheology, Clin. Hemorheol., 6, 439 (1986)

[14] Meiselman HJ, Scand. J. Clin. Lab. Invest., 41, 27 (1981)

[15] Musielak M, Clin. Hemorheol. Microcirc., 42, 47 (2009)

[16] Nash GB, Meiselman HJ, Ann. N. Y. Acad. Sci., 416, 255 (1983)

[17] Nemeth N, Alexy T, Furka A, Baskurt OK, Meiselman HJ, Furka I, Miko I, Biorheology, 46, 155 (2009)

[18] Nemeth N, Baskurt OK, Meiselman HJ, Kiss F, Uyuklu M, Hever T, Sajtos E, Kenyeres P, Toth K, Furka I, Miko I, Korea-Aust. Rheol. J., 21(2), 127 (2009)

[19] Nemeth N, Baskurt OK, Meiselman HJ, Furka I, Miko I, Korea-Aust. Rheol. J., 21(3), 155 (2009)

[20] Nemeth N, Baskurt OK, Meiselman HJ, Miko I, Clin. Hemorheol. Microcirc., 43, 257 (2009)

[21] Nemeth N, Kiss F, Furka I, Miko I, Gender differences of blood rheological parameters in laboratory animals. Clin. Hemorheol. Microcirc. . accepted for publication. (2010)

[22] Pfafferott C, Nash GB, Meiselman HJ, Biophys. J., 47, 695 (1985)

[23] Schmid-Schonbein H, Wells R, Science, 165, 288 (1969)

[24] Shin S, Ku Y, Park MS, Suh JS, Cytometry Part B (Clinical Cytometry), 65, 6 (2005)

[25] Stoltz JF, Singh M, Riha P, eds, Microrheological parameters, in: Hemorheology in Practice, IOS Press, Amsterdam, The Netherlands, pp. 27-52. (1999)

[26] Windberger U, Baskurt OK, Comparative hemorheology, in: Handbook of Hemorheology and Hemodynamics, Baskurt OK, Hardeman MR, Rampling MW, Meiselman HJ, eds, IOS Press, Amsterdam, The Netherlands, pp. 267-285.

[27] Yerer MB, Aydogan S, Comu FM, Clin. Hemorheol. Microcirc., 39, 423 (2008)