To investigate the dependence of filter ripening on particle size and surface charge, multiple experiments were conducted under different particle destabilization conditions including pH control, alum, and polymer destabilization. Laboratory-scale filtration experiments were performed at a filtration velocity of 5m/h using spherical glass beads with mean diameter of 0.55mm as collectors. Particle suspensions with a broad size distribution and a 1.7 mu m mean particle size were filtered through a 10cm depth filter column. Better initial solids removal was confirmed under favorable particle and collector conditions (i.e., under smaller surface charge), but better initial particle removal does not necessarily mean better overall particle removal efficiency. It was shown that changes of the particle size distribution (PSD) in the effluent can significantly influence overall particle removal efficiency. Chemical parameters such as zeta potential can be important during the initial stage of filtration, but their importance can decrease over time depending on the specific chemical conditions. The influent PSD and the removal of certain size particles during the initial stage of filtration can significantly influence ripening, which in turn, can influence the overall particle removal efficiency.