Seabuckthorn pulp oil was fractionated using a crystallization process with acetone under controlled cooling rate of 0.25 degrees C/min without agitation at different crystallization temperatures ranging from -15 to 15 degrees C. The obtained liquid (LF) and solid (SF) fractions were analyzed for their fatty acid and triacylglycerol compositions and their melting profiles were characterized. Fractionation at -15 degrees C yielded about 20% of LF where palmitoleic acid represented 53% of total lipids. The SF fraction was mainly rich in palmitic acid. LF were richer in triacylglycerol with acyl carbon numbers of 50 and 52 (C50 and C52) than SF, which contained a higher amount of C48. The melting curves of LF and SF showed multiple endothermic transitions.