Tetraaza-14-crown-4 and oleic acid was successfully applied for transport of Cu(II) in chloroform bulk liquid membrane. The uphill moving of Cu(II) during the liquid membrane transport process has occurred. The main effective variable such as the type of the metal ion acceptor in the receiving phase and its concentration, tetraaza-14-crown-4 and oleic acid concentration in the organic phase on the efficiency of the ion-transport system were examined. By using L-cysteine as a metal ion acceptor in the receiving phase, the maximum amount of copper (II) transported across the liquid membrane was achieved to 96 +/- 1.5% after 140 minutes. The selectivity of copper ion transport from the aqueous solutions containing Pb2+, Tl+, Ag+, Co2+, Ni2+, Mg2+, Zn2+, Hg2+, Cd2+ and Ca2+ ions were investigated. In the presence of CH3COONH4 and Na4P2O7 as suitable masking agents in the source phase, the interfering effects of Pb+2 and Cd2+ were diminished drastically.