A light or pH dual-responsive reactivated biointerface is fabricated using of photocontrolled reversible inclusion and exclusion reactions between photoresponsive azobenzene-containing self-assembled monolayer and pH-responsive poly(acrylic acid) polymer grafted with cyclodextrins. The dual-controlled reactivated biointerface can be employed for reversible immobilization of rectos protein-Cytochrome c, triggered by dual external stimuli-light and pH.