PRODUCTION OF BERBERINE IN IMMOBILIZED PLANT CELL CULTURE OF<I> Thalictrum rugosum</I>

CHOI JW

Korean Journal of Chemical Engineering, Vol.7, No.3, 226-232, July, 1990

PRODUCTION OF BERBERINE IN IMMOBILIZED PLANT CELL CULTURE OF Thalictrum rugosum

Thalictrum rugosum cells were immobilized in calcium alginate, where they continued to live with their biological activity. The immobilized living cells performed the production of berberine in both shake flasks and an airlift bioreactor. Berberine formation was growth associative and most of the berberine produced was stored intracellularly. Rapid hydrolysis of sucrose and preference of glucose over fructose during the growth stage was observed. Phosphate-deficient media increased berberine production and prevented the dissolution of alginate beads. The behavior of immobilized cells grown in an airlift reactor was compared with that of the corresponding shake flask culture with respect to growth and berberine production. The rate of cell growth and berberin production in an airlift reactor operation was higher than those in packed-column reactor operation due to a better oxygen transfer.

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[1] Hulst AC, Tramper J, Enzyme Microb. Technol., 11, 546 (1989)

[2] Panda AK, Mishra S, Bisaria VS, Bhojwani S, Enzyme Microb. Technol., 11, 386 (1989)

[3] Prenosil J, Pedersen H, Enzyme Microb. Technol., 5, 323 (1983)

[4] Pedersen H, Chin CK, Venkatasubramanian K, "Biocatalysis in Agricultural Biotechnology," (ed. by Whitaker, J.R. and Sonnet, P.E.), p. 193, American Chemical Society, Washingtn, D.C. (1989)

[5] Hong YC, Labuza TP, Harlander SK, Biotechnol. Prog., 5, 137 (1989)

[6] Scragg AH, Enzyme Microb. Technol., 12, 82 (1990)

[7] Scragg AH, Enzyme Microb. Technol., 12, 82 (1990)

[8] Scragg AH, Morris P, Allan EJ, Bond P, Fowler MW, Enzyme Microb. Technol., 9, 619 (1987)

[9] Nakagawa K, Konagai A, Fukui H, Tabata M, Plant Cell Rep., 3, 254 (1984)

[10] Choi JW, Ph.D. Dissertation, Rutgers Univ., New Brunswick, U.S.A. (1990)

[11] Kim DI, Ph.D. Dissertation, Rutgers Univ., New Brunswick, U.S.A. (1989)

[12] Berlin J, Mollenschott C, Wray V, Biotechnol. Lett., 10, 193 (1988)

[13] Smart NJ, Fowler MW, J. Exp. Botany, 35, 531 (1984)

[14] Furuya T, Yoshikaua T, Taira M, Phytochem., 23, 999 (1984)