In this review, we summarize the present understanding of the Mycobacterium tuberculosis transcriptome and catalog both experimentally verified findings and computationally derived predictions. We also provide a new analysis for a range of discoveries by comparing the results of previously independent research papers. Bringing these data together and improving their accessibility should help catalyze further discoveries. This minireview also provides some general insights that may be valuable to those working to characterize the transcriptome of less-studied prokaryotes.