IN SITU BERBERINE SEPARATION WITH IMMOBILIZED ADSORBENT IN CELL SUSPENSION CULTURES OF Thalictrum rugosum

Choi JW

Korean Journal of Chemical Engineering, Vol.9, No.3, 128-134, July, 1992

The use of suspended and alginate-entrapped XAD-7, polycarboxyl ester resin, for the in situ separation of berberine, isoquinoline alkaloid, produced from plant cell culture of Thalictrum rugosum was investigated. XAD-7 could adsorb the berberine and the amount of berberine adsorbed on XAD-7 depended on pH. The neutral form of berberine was adsorbed onto XAD-7 and the adsorption isotherm for berberine showed a Langmuir-type appearance. In situ berberine removal enhanced the production of secondary metabolites in cell suspension culture of Thalictrum rugosum. Addition of XAD-7 at the exponential phase of cell growth was the most effective for enhancement of berberine production. In chitosan-treated cell culture to permeabilize intracellular berberine, berberine secretion was significantly accelerated by addition of alginate-entrapped XAD-7 at the stationary phase of cell growth and thus more than 70% of the produced berberine could be adsorbed to alginate-entrapped XAD-7.

[References]

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[2] Payne GF, Payne NN, Biotechnol. Lett., 10, 187 (1988)

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[4] Robins RJ, Rhodes MJC, Appl. Microbiol. Biotechnol., 24, 35 (1986)

[5] Deno H, Suga C, Morimoto T, Fujita Y, Plant Cell Rep., 6, 197 (1987)

[6] Cormier F, Ambid C, Plant Cell Rep., 6, 427 (1987)

[7] Berlin J, Witte L, Schubert W, Wray V, Phytochem., 23, 1277 (1984)

[8] Hooker BS, Lee JM, Plant Cell Rep., 8, 546 (1990)

[9] Payne GF, Shuler ML, Biotechnol. Bioeng., 15, 633 (1985)

[10] Paleos J, J. Colloid Interface Sci., 31, 7 (1969)

[11] Payne GF, Shuler ML, Biotechnol. Bioeng., 31, 922 (1988)

[12] Asada M, Shuler ML, Appl. Microbiol. Biotechnol., 30, 475 (1989)

[13] Fowler MW, J. Chem. Technol. Biotechnol., 32, 338 (1982)

[14] Fowler MW, Trends Biotechnol., 14, 214 (1986)

[15] Zenk MA, Rueffer M, Amann M, DeusNeumann B, J. Natural Plants, 48, 725 (1985)

[16] Robison RC, Cha DY, Biotechnol. Prog., 1, 18 (1985)

[17] Choi JW, Ph.D. Thesis, Rutgers Univ., New Brunswick, U.S.A. (1990)

[18] Nigam SC, Siahpush AR, Wang HY, AIChE J., 36, 1239 (1990)

[19] Young DH, Kauss H, Plant Pysiol., 73, 698 (1983)