In order to separate small magnetizable particles down to the micro-and nanometer scale, high-gradient magnetic separation is a well-established process. The superposition of magnetic filtration with centrifugation, called magnetic field-enhanced centrifugation, permits continuous separation. The separation efficiency of magnetic filters and the prediction of the efficiency is described. The separation efficiency of one single stage could be enhanced from 51 to 78% by increasing the wire number and improving the wire cross section. The separation efficiency on different particle sizes at different filter stage numbers and comparison to the prediction of separation efficiency based on magnetic forces and the fluid drag force is demonstrated.