EXPERIMENTAL STUDIES ON PHASE EQUILIBRIA OF PEG-WATER-DEXTRAN/AMMONIUM SULFATE SYSTEMS AND PARTITIONING OF ALBUMIN INTO TWO WATER-CONTINUOUS PHASES

Kim BK; Ban YB; Kim JD

Korean Journal of Chemical Engineering, Vol.9, No.4, 219-224, October, 1992

Kim BK, Ban YB, Kim JD

The clouding points and equilibrium concentrations of PEG/dextran/water and PEG/ammonium sulfate/water systems were experimentally determined for different molecular weights of PEG by titration method and direct determination of concentrations. In phase diagrams of PEG-water with ammonium sulfate or dextran, the addition of salt or dextran induced the phase separation of PEG-rich phases near the PEG-lean phases having the different partitioning of PEG. The concentrations of PEG in PEF-rich phases increase as the amounts of PEG or salt and dextran increase, while the concentrations of PEG in aqueous media decease in any cases. the higher the molecular weight of peg has, the wider the two-phase regions are. In dextran DT10 systems, the partition coefficients of egg albumin into PEG-lean phases increase with concentrations of dextran and the molecular weight of PEG. In ammonium sulfate systems, the partitioning coefficients showed a maxi-mum, having lower partitioning at the very high and low concentration of salts. It is also observed that as the amounts of albumin increase, the partitioning of albumin into PEG-lean phase increases.

[References]

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Abbot NL, Hatton A, Chem. Eng. Prog., Aug., 31 (1988)
Albertsson PA, Ryden J, J. Colloid Interface Sci., 37, 219 (1971)
Lou J, Acta. Chem. Scand, B37, 241 (1983)
Albertsson PA, Arch. Biochem. Biophys., 1, 264 (1962)
Hustedt H, Kroner KH, Stach W, Kula MR, Biotechnol. Bioeng., 20, 1989 (1978)
Albertsson PA, Sasakawa S, Walter H, Nature, 228, 1329 (1970)
Alam S, Ph.D. Thesis, Illinois Institute of Technology, Chicago, Illinois (1988)
Bronsted JN, Z. Phys. Chem. A, 155, 257 (1931)
Bronsted JN, Warming E, Z. Phys. Chem., 155, 343 (1931)
Gorson D, Biochim. Biophys. Acta, 602, 269 (1980)
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King R, Blanch HW, Prausnitz JM, AIChE J., 34, 1585 (1988)
Kim CW, Ph.D. Thesis, Massachusetts Institute of Technology, Cambridge, MA (1986)
Kang CH, Sandler SI, Fluid Phase Equilib., 38, 245 (1987)
Haynes CA, Blanch HW, Prausnitz JM, Fluid Phase Equilib., 53, 463 (1989)
Haynes CA, Beynon RV, King RS, Blanch HW, Prausnitz JM, J. Phys. Chem., 93, 5612 (1989)
Huggins ML, J. Phys. Chem., 46, 151 (1942)
Flory PJ, J. Chem. Phys., 12, 425 (1944)
Flory PJ, "Principles of Polymer Chemistry," Cornell Univ. Press (1971)
Kim YC, Kim JD, Fluid Phase Equilib., 41, 229 (1988)
Kim JD, Kim YC, Ban YB, Fluid Phase Equilib., 53, 323 (1989)
Kim BK, Master Thesis, KAIST, Seoul (1989)
Ban YB, Kim JD, Polym. Prepr., 30, 94 (1989)
Ban YB, Kim JD, "Computer Simulation of Polymers," ed. by Roe, R.J., Prentice Hall (1991)
Shultz AR, Flory PJ, J. Am. Chem. Soc., 74, 4760 (1952)

[Referenced By]

[1] Albertsson PA, "Partition of Cell Particles and Macromolecules," 3rd ed., Wiley Interscience, N.Y. (1986)

[2] Baskir JN, Hatton TA, Suter UW, Biotechnol. Bioeng., 34, 541 (1989)

[3] Walter H, Brooks DE, Fisher D, "Partitioning in Aqueous Two Phase Systems," Academic Press, London (1985)

[4] Abbot NL, Hatton A, Chem. Eng. Prog., Aug., 31 (1988)

[5] Albertsson PA, Ryden J, J. Colloid Interface Sci., 37, 219 (1971)

[6] Lou J, Acta. Chem. Scand, B37, 241 (1983)

[7] Albertsson PA, Arch. Biochem. Biophys., 1, 264 (1962)

[8] Hustedt H, Kroner KH, Stach W, Kula MR, Biotechnol. Bioeng., 20, 1989 (1978)

[9] Albertsson PA, Sasakawa S, Walter H, Nature, 228, 1329 (1970)

[10] Alam S, Ph.D. Thesis, Illinois Institute of Technology, Chicago, Illinois (1988)

[11] Bronsted JN, Z. Phys. Chem. A, 155, 257 (1931)

[12] Bronsted JN, Warming E, Z. Phys. Chem., 155, 343 (1931)

[13] Gorson D, Biochim. Biophys. Acta, 602, 269 (1980)

[14] Brooks DE, Sharp KE, Fisher DE, "Partitioning in Aqueous Two-Phase Systems," Walter, H., Brooks, D.E. and Fisher, D., eds., Academic, New York (1985)

[15] King R, Blanch HW, Prausnitz JM, AIChE J., 34, 1585 (1988)

[16] Kim CW, Ph.D. Thesis, Massachusetts Institute of Technology, Cambridge, MA (1986)

[17] Kang CH, Sandler SI, Fluid Phase Equilib., 38, 245 (1987)

[18] Haynes CA, Blanch HW, Prausnitz JM, Fluid Phase Equilib., 53, 463 (1989)

[19] Haynes CA, Beynon RV, King RS, Blanch HW, Prausnitz JM, J. Phys. Chem., 93, 5612 (1989)

[20] Huggins ML, J. Phys. Chem., 46, 151 (1942)

[21] Flory PJ, J. Chem. Phys., 12, 425 (1944)

[22] Flory PJ, "Principles of Polymer Chemistry," Cornell Univ. Press (1971)

[23] Kim YC, Kim JD, Fluid Phase Equilib., 41, 229 (1988)

[24] Kim JD, Kim YC, Ban YB, Fluid Phase Equilib., 53, 323 (1989)

[25] Kim BK, Master Thesis, KAIST, Seoul (1989)

[26] Ban YB, Kim JD, Polym. Prepr., 30, 94 (1989)

[27] Ban YB, Kim JD, "Computer Simulation of Polymers," ed. by Roe, R.J., Prentice Hall (1991)

[28] Shultz AR, Flory PJ, J. Am. Chem. Soc., 74, 4760 (1952)