By precisely fixing an inner cooling capillary to the axial center of a quartz tube, a new and better wide-bore electrophoresis (WBE) system was built and has shown great potential in load capacity and separation efficiency. Further, a novel two-dimensional hybrid of HPLC and WBE system (HPLC WBE) was developed with an injection valve as the interface. High-resolution and rapid analysis speed were observed in both the WBE system and the 2D HPLC-WBE system. Further work is currently underway.