Glycerin에 수화된 인지질 라멜라 액정상의 구조에 미치는 Ceramide 3의 영향 : 구조적 및 열적거동

정관영; 이동규; Kwan-Young Jeong; Dong-Kyu Lee

Applied Chemistry for Engineering, Vol.21, No.6, 603-609, December, 2010

Influence of Ceramide III on the Structure of a Phospholipid Lamellar Liquid Crystalline Phase Hydrated in Glycerin : Structural and Thermal Behaviors

정관영, 이동규 ^{1, †}

한국콜마 피부과학연구소
¹충북대학교 공업화학과

Kwan-Young Jeong, and Dong-Kyu Lee^{1, †}

Skin Research Institute, Korea Kolmar Corporation, Chung-Nam 339-850, Korea
¹Department of Industrial Engineering Chemistry, College of Engineering, Chungbuk National University, Cheong-ju 361-763, Korea

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초록

수첨 인지질의 하나인 distearoylphosphatidylcholin (DSPC)을 주로 하면서 피부보습에 도움을 주는 용매인 glycerin (GLY)에 의해 팽윤되어 형성된 라멜라 액정상 구조에 피부장벽을 구성하는 지질 중 하나로서 ceramide 3 (CER3)이 함께 배향될 때 나타나는 함량 의존적 거동을 DSC, XRDs, PM 및 Cryo-SEM에 의해 고찰하였다. CER3은 매우 결정성이 높지만, DSPC와의 구조적인 유사성을 가지고 있어 DSPC 20.0 wt% 함량대비 7.0 wt%까지는 탈리 없이 배향되지만, 그 이상을 배향시킬 경우 CER3이 액정 내에서 탈리되어 자체로 GLY에 팽윤되어 또 다른 액정상을 형성하는 것을 XRD의 특성피크 발현과 DSC의 공융점 거동을 통해 확인할 수 있었다. 또한 CER3이 액정 내에 배향되면서 DSPC만으로 이루어진 액정에서 보이는 SPP패턴뿐만 아니라, 피부 각질층에서 주로 나타나는 LPP패턴도 SAXD분석을 통해 확인되어, CER3을 적용시 인간피부와 보다 흡사한 액정구조를 형성함을 확인하였다. Cryo-SEM을 이용하여 DSPC만으로 이루어진 액정과 여기에 CER3이 도입된 액정을 비교한 결과, CER3은 액정의 구조를 보다 두텁고, 견고하게 함을 알 수 있었다.

This paper aims to investigate the lyotropic behaviors of DSPC and CER3 when they are swollen by GLY as a solvent. The analyses were carried out on DSC, XRDs, PM, and Cryo-SEM. CER3 which has its high crystallinity and structural similarity with DSPC was well arranged up to 7.0 wt% in comparison to 20 wt% DSPC without any separation, but it was separated from the liquid crystalline (LC) phase to form another crystalline phase with the expression of its characteristic peak in XRDs and eutectic thermal behavior in DSC. Introducing CER3, two types of patterns were shown in XRD spectra; one is SPP expressed in a normal LC and another is LPP expressed in human skin SC. Therefore, it was confirmed that the incorporation of CER3 makes LC structure more similar to human skin. In Cryo-SEM study, it was shown that CER3 makes LC structure thicker and denser.

Keywords:ceramide 3;distearoylphosphatidylcholin;periodicity phase;lamellar

[References]

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Nicolazzo JA, Morgan TM, Reed BL, Finnin BC, J.Controlled Release., 103, 577 (2005)
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Fang JY, Hwang TL, Leu YL, Int. J. Pharm., 250, 313 (2003)
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Brown GH, Wolken JJ, Liquid Crystals and Biological Structures, Academic Press, New York. (1979)
Blumstein A, Blumstein RB, Clough SB, Hsu EC, Macromolecules., 8, 73 (1975)
Wertz PW, Miethke MC, Long SA, Strauss JS, Downing DT, J. Invest. Dermatol., 84, 410 (1985)
Robson KJ, Stewart ME, Michelsen S, Lazo ND, Downing DT, J. Lipid. Res., 35(2060) (1994)
Stewart ME, Downing DT, J. Lipid. Res., 4, 1434 (1999)
Ponec M, Weerheim A, Lankhorst P, Wertz PW, J. Invest. Dermatol., 120, 581 (2003)
Zbytovska J, Kiselev MA, Funari SS, Garamus VM, Wartewig S, Neubert R, Chemistry and Physics of Lipids., 138, 69 (2005)
Bouwstra JA, Gooris GS, van der Spek JA, Bras WJ, Invest. Dermatol., 97, 1005 (1991)
Bouwstra JA, Gooris GS, van der Spek JA, Bras W, J. Invest. Dermatol., 96 (1006)
Bouwstra JA, Gooris GS, Bras W, Dowing DT, J. Lipid Res., 36, 685 (1995)
Bouwstra JA, Gooris GS, Cheng K, Weerheim A, Bras W, J. Lipid Res., 37, 999 (1996)
Bouwstra JA, Gooris GS, Dubbelaar FER, Ponec M, J. Lipid Res., 42, 1759 (2001)
Bouwstra JA, Gooris GS, Dubbelaar FER, Ponec M, J. Invest. Dermatol., 118, 606 (2002)
de Jager MW, Gooris GS, Dolbnya IP, Bras W, Ponec M, Bouwstra JA, Chem. Phys. Lipids., 124, 123 (2003)

[Referenced By]

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[1] Sheth NV, Freeman DJ, Higuchi WI, Spruance SL, Int. J. Pharm., 28, 201 (1986)

[2] Nicolazzo JA, Morgan TM, Reed BL, Finnin BC, J.Controlled Release., 103, 577 (2005)

[3] Lim PFC, Liu XY, Kang L, Ho PCL, Chan YW, Chan SY, Int. J. Pharm., 311, 157 (2006)

[4] Fang JY, Hwang TL, Leu YL, Int. J. Pharm., 250, 313 (2003)

[5] Luzzati V, Husson F, J. Cell, Biol., 12, 207 (1962)

[6] Brown GH, Wolken JJ, Liquid Crystals and Biological Structures, Academic Press, New York. (1979)

[7] Blumstein A, Blumstein RB, Clough SB, Hsu EC, Macromolecules., 8, 73 (1975)

[8] Wertz PW, Miethke MC, Long SA, Strauss JS, Downing DT, J. Invest. Dermatol., 84, 410 (1985)

[9] Robson KJ, Stewart ME, Michelsen S, Lazo ND, Downing DT, J. Lipid. Res., 35(2060) (1994)

[10] Stewart ME, Downing DT, J. Lipid. Res., 4, 1434 (1999)

[11] Ponec M, Weerheim A, Lankhorst P, Wertz PW, J. Invest. Dermatol., 120, 581 (2003)

[12] Zbytovska J, Kiselev MA, Funari SS, Garamus VM, Wartewig S, Neubert R, Chemistry and Physics of Lipids., 138, 69 (2005)

[13] Bouwstra JA, Gooris GS, van der Spek JA, Bras WJ, Invest. Dermatol., 97, 1005 (1991)

[14] Bouwstra JA, Gooris GS, van der Spek JA, Bras W, J. Invest. Dermatol., 96 (1006)

[15] Bouwstra JA, Gooris GS, Bras W, Dowing DT, J. Lipid Res., 36, 685 (1995)

[16] Bouwstra JA, Gooris GS, Cheng K, Weerheim A, Bras W, J. Lipid Res., 37, 999 (1996)

[17] Bouwstra JA, Gooris GS, Dubbelaar FER, Ponec M, J. Lipid Res., 42, 1759 (2001)

[18] Bouwstra JA, Gooris GS, Dubbelaar FER, Ponec M, J. Invest. Dermatol., 118, 606 (2002)

[19] de Jager MW, Gooris GS, Dolbnya IP, Bras W, Ponec M, Bouwstra JA, Chem. Phys. Lipids., 124, 123 (2003)