Silver nanoparticle-modified composite electrodes were prepared by electroless forming silver nanoparticles in carbon black dispersed electroless silver plating solution, and then incorporating the silver nanoparticle/carbon black mixture in a polystyrene matrix. The electrooxidation of glucose in 0.1 MNaOH was studied by using cyclic voltammetry. The electrode has surface-confined nanoparicles showing effective catalytic behavior in the studies of glucose oxidation. It showed stepwise electrocatalytic oxidation behavior clearly without suppression by high. background redox current caused by inner layer silver of disk-type silver electrodes. Initially the glucose was catalytically oxidized by AgO, and then the reaction glucose oxidation intermediates with Ag2O were followed stepwisely. Based on the cyclic voltammetric results, pulsed amperometric detection parameters for flow injection analysis were optimized for sensitive detection of glucose. (C) 2009 Elsevier Ltd. All rights reserved.