An insulin-imprinted polymer was synthesized over the surface of vinyl group functionalized multiwalled carbon nanotubes using phosphotidylcholine-containing functional monomer and cross-linker Phosphotidylcholine is a major component of all biological membrane its incorporation in polymer backbone assures water-compatibility bio-compatibility and specificity to molecularly imprinted nanomaterials without any cross-reactivity or interferences from biological sample matrices An electrochemical sensor fabricated by modifying multiwalled carbon nanotubes-molecularly imprinted polymer onto the pencil graphite electrode was used for trace level detection of insulin in aqueous blood serum and pharmaceutical samples (detection limit 0 0186 nmol L-1 S/N = 3) by differential pulse anodic stripping voltammetry Additional cyclic voltammetry (stripping mode) and chronocoulometry experiments were performed to explore electrodics and kinetics of electro-oxidation of insulin (C) 2010 Elsevier Ltd All rights reserved