In the present work, a novel sensor for luminol electrochemiluminescence (ECL) was constructed on the base of a C-doped titanium oxide amorphous semiconductor electrode. The morphology, structural and electrochemical properties of the electrode was characterized by X-Ray diffraction, X-Ray photoelectron spectroscopy and electrochemical methods. The ECL behavior of luminol excited by hot electrons injected from C-doped oxide film-covered electrodes in aqueous medium has been investigated in B-R buffer solution (pH = 9) when linear sweep cyclic voltammetry (CV) was applied. Two ECL peaks were observed at -1.0 V (vs. Ag/AgCl, reduction process) and -0.75 V (vs. Ag/AgCl, oxidation process). The possible mechanism was discussed. The C-doped Ti oxide electrode shows excellent properties for sensitive determination of luminol with good reproducibility and stability. The linear response of luminol was in the range of 1 x 10(-8) to 9 x 10(-8) mol/L with the detection limit of 3 x 10(-9) mol/L (S/N = 3). Since luminol is one of the most useful ECL probe, many bioactive compounds which can be labeled by luminol are able to be detected by using the proposed method. (C) 2010 Elsevier Ltd. All rights reserved.