실크피브로인을 함유한 PLGA 하이브리드 필름이 슈반세포의 부착과 증식에 미치는 영향

김혜린; 유한나; 박현진; 김용기; 이동원; 강영선; 강길선; Hye Lin Kim; Hanna Yoo; Hyun Jin Park; Yong Gi Kim; Dongwon Lee; Young Sun Kang; Gilson Khang

Polymer(Korea), Vol.35, No.1, 7-12, January, 2011

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실크피브로인을 함유한 PLGA 하이브리드 필름이 슈반세포의 부착과 증식에 미치는 영향

Effect of PLGA/Silk Fibroin Hybrid Film on Attachment and Proliferation of Schwann Cells

김혜린, 유한나, 박현진, 김용기, 이동원 , 강영선¹, 강길선 ^†

전북대학교 BIN 융합공학과, 고분자 나노공학과
¹건국대학교 의생명과학연구원

Hye Lin Kim, Hanna Yoo, Hyun Jin Park, Yong Gi Kim, Dongwon Lee , Young Sun Kang¹, and Gilson Khang^†

Department of BIN Fusion Technology & Department of PolymerㆍNano Science & Technology, Chonbuk National University, 664-14, Dukjin Ku, Jeonju 561-756, Korea
¹Institute of Biomedical Science and Technology, Konkuk University, Hwayang, Gwangjin, Seoul 143-130, Korea

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초록

PLGA는 미국 식품의약품안전청의 승인을 받은 합성고분자로서 생체재료로 널리 쓰이며, 실크피브로인은 세포친화성 및 우수한 기계적 특성으로 세포의 조직성장을 지지한다. 본 연구에서는 PLGA/실크피브로인 0, 10, 20, 40 및 80 wt% 하이브리드 필름을 제조하였고 함량별 슈반세포의 최적환경을 설정하고자 하였다. DSC결과 PLGA/실크피브로인 필름이 in vivo 적용시 열적으로 안정할 것으로 사료되었다. 접촉각측정을 통해 PLGA/실크피브로인 10 및 20 wt% 필름이 세포 분화 및 증식에 적합할 것으로 사료되며 RT-PCR과 SEM 결과로부터 PLGA에 10 및 20 wt%의 실크피브로인을 함유한 필름에서 슈반세포의 우수한 부착거동 및 형태유지를 확인하였다. 또한 WST 결과 10 wt% 실크피브로인 함량에서 높은 증식률이 확인되었다. 결과적으로 PLGA/실크피브로인 10 및 20 wt% 필름이 세포에 알맞은 상호관계를 제공하여 긍정적으로 작용함을 확인하였다.

Poly(lactic-co-glycolic acid) (PLGA) is a biodegradable synthetic polymer with acceptable mechanical strength and well-controlled degradation rate. Also, it can be easily fabricated into many shapes. Silk fibroin contains powerful bioactive molecules. We fabricated natural/synthetic hybrid films using 0, 10, 20, 40 and 80 wt% of silk fibroin. Schwann cells (SCs) were seeded on PLGA/silk fibroin hybrid films and confirmed the effects of adhesion and proliferation on SCs according to the content of silk fibroin. In this study, we confirmed PLGA/silk fibroin hybrid film containing 40% and 80% of silk fibroin interrupted adhesion and proliferation of SCs. Films containing 10% and 20% of silk, however, provided suitable environment for growth and proliferation of SCs. These results suggest that silk fibroin provides suitables surface to neural cells and its proper content provides proper culture conditions to improve cell adhesion and proliferation.

Keywords:silk fibroin;poly(lactic-co-glycolic acid)(PLGA);Schwann cell;hybrid film;tissue engineering

[References]

Zhang N, Yan H, Wen X, Brain Res. Rev., 49, 48 (2005)
Palace J, J. Neurol. Sci., 265, 21 (2008)
Jandial R, Singec I, Duenas VJ, Ho AL, Levy ML, Snyder EY, Inter. Congress Series., 1302, 154 (2007)
Mills CD, Allchorne AJ, Griffin RS, Mol. Cell.Neurosci., 36, 185 (2007)
Nissinen M, Honkanen H, Lahti O, Kangas M, Peltonen J, Peltonen S, Mol. Cell. Neurosci., 37, 568 (2008)
Imaizumi T, Lankford KL, Kocsis JD, Brain Res., 854, 70 (2000)
Doherty GH, Oldreive C, Harvey J, Neurosci., 154, 1297 (2008)
Yang DP, Zhang DP, Mak KS, Mol. Cell. Neurosci., 38, 80 (2008)
Dahlin LB, Current Orthopaedics., 22, 9 (2008)
Cheng C, Webber CA, Wang J, Xu Y, Martinez JA, Liu WQ, McDonald D, Guo GF, Nguyen MD, Zochodne DW, Exp. Neurol., 212, 358 (2008)
Khang G, Kim SK, Hong KD, Tissue Eng. Regen. Med., 1, 136 (2004)
Caddick J, Kingham PJ, Gardiner NJ, Glia., 54, 840 (2006)
Movaghara B, Tiraihia T, Mesbah-Naminb SA, Cell Biol. Int., 30, 569 (2006)
Minoura N, Aiba S, Higuchi M, Gotoh Y, Tsukada M, Imai Y, Biochem. Biophys. Res. Commun., 208, 511 (1995)
Altman GH, Diaz F, Jakuba C, Calabro T, Horan RL, Chen J, Lu H, Richmond J, Kaplan DL, Biomaterials., 24, 401 (2003)
Chen J, Altman GH, Karageorgiou V, Horan R, Collette A, Volloch V, J. Biomed. Mater. Res., 67, 559 (2003)
Jin HJ, Kaplan DL, Nature., 424, 1057 (2003)
Jin HJ, Fridrikh SV, Rutledge GC, Kaplan DL, Biomacromolecules, 3(6), 1233 (2002)
Meinel L, Betz O, Fajardo R, Hofmann S, Nazarian A, Bone., 39, 4 (2006)
Uebersaxa L, Mattottia M, Papaloizosb M, Biomaterials., 28, 30 (2007)
Garcia-Fuentesa M, Meinela AJ, Hilbeb M, Meinela L, Merkle HP, Biomaterials., 30, 28 (2009)
Kim EJ, Song JH, Kim MS, Tissue Eng. Regen. Med., 1, 41 (2004)
Khang G, Jeon EK, Rhee JM, Lee I, Lee SJ, Lee HB, Macromol. Res., 11(5), 334 (2003)
Kingham PJ, Kalbermatten DF, Mahay D, Armstrong SJ, Wiberg M, Terenghi G, Exp. Neurol., 207, 267 (2007)
Jang JW, Park KS, Kim SH, Tissue Eng. Regen. Med., 2, 34 (2005)
Khang G, Jeon JH, Cho JC, Lee HB, Polym.(Korea), 23(3), 471 (1999)
Khang G, Kim MS, Cho SH, Tissue Eng. Regen. Med., 1, 9 (2004)
Sagnella S, Anderson E, Sanabria N, Marchant RE, Marchant K, Tissue Eng., 11, 226 (2005)
Iwasaki Y, Sawada S, Nakabayashi N, Khang G, Lee HB, Ishihara K, Biomaterials., 20, 2185 (1999)
Khang GS, Kim MS, Min BH, Lee IW, Rlee JM, Lee HB, Tissue Eng. Regen. Med., 3, 376 (2006)
Morrissey TK, Leitman NK, Bunge RP, J. Neurosci., 11, 2433 (1991)
Liu H, Fan H, Wang Y, Toh SL, Goh JC, Biomaterials., 29, 662 (2008)
Park KS, Kim SM, Kim MS, Lee I, Rhee JM, Lee HB, Khang G, Polym.(Korea), 30(5), 445 (2006)
Oh YA, Kim SH, Lee SJ, Yoo JJ, van Dyke M, Rhee JM, Khang G, Polym.(Korea), 32(5), 403 (2008)
Li C, Vepari C, Jina H, Kim HJ, Kaplan DL, Biomaterials., 27, 3115 (2006)

[Referenced By]

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[1] Zhang N, Yan H, Wen X, Brain Res. Rev., 49, 48 (2005)

[2] Palace J, J. Neurol. Sci., 265, 21 (2008)

[3] Jandial R, Singec I, Duenas VJ, Ho AL, Levy ML, Snyder EY, Inter. Congress Series., 1302, 154 (2007)

[4] Mills CD, Allchorne AJ, Griffin RS, Mol. Cell.Neurosci., 36, 185 (2007)

[5] Nissinen M, Honkanen H, Lahti O, Kangas M, Peltonen J, Peltonen S, Mol. Cell. Neurosci., 37, 568 (2008)

[6] Imaizumi T, Lankford KL, Kocsis JD, Brain Res., 854, 70 (2000)

[7] Doherty GH, Oldreive C, Harvey J, Neurosci., 154, 1297 (2008)

[8] Yang DP, Zhang DP, Mak KS, Mol. Cell. Neurosci., 38, 80 (2008)

[9] Dahlin LB, Current Orthopaedics., 22, 9 (2008)

[10] Cheng C, Webber CA, Wang J, Xu Y, Martinez JA, Liu WQ, McDonald D, Guo GF, Nguyen MD, Zochodne DW, Exp. Neurol., 212, 358 (2008)

[11] Khang G, Kim SK, Hong KD, Tissue Eng. Regen. Med., 1, 136 (2004)

[12] Caddick J, Kingham PJ, Gardiner NJ, Glia., 54, 840 (2006)

[13] Movaghara B, Tiraihia T, Mesbah-Naminb SA, Cell Biol. Int., 30, 569 (2006)

[14] Minoura N, Aiba S, Higuchi M, Gotoh Y, Tsukada M, Imai Y, Biochem. Biophys. Res. Commun., 208, 511 (1995)

[15] Altman GH, Diaz F, Jakuba C, Calabro T, Horan RL, Chen J, Lu H, Richmond J, Kaplan DL, Biomaterials., 24, 401 (2003)

[16] Chen J, Altman GH, Karageorgiou V, Horan R, Collette A, Volloch V, J. Biomed. Mater. Res., 67, 559 (2003)

[17] Jin HJ, Kaplan DL, Nature., 424, 1057 (2003)

[18] Jin HJ, Fridrikh SV, Rutledge GC, Kaplan DL, Biomacromolecules, 3(6), 1233 (2002)

[19] Meinel L, Betz O, Fajardo R, Hofmann S, Nazarian A, Bone., 39, 4 (2006)

[20] Uebersaxa L, Mattottia M, Papaloizosb M, Biomaterials., 28, 30 (2007)

[21] Garcia-Fuentesa M, Meinela AJ, Hilbeb M, Meinela L, Merkle HP, Biomaterials., 30, 28 (2009)

[22] Kim EJ, Song JH, Kim MS, Tissue Eng. Regen. Med., 1, 41 (2004)

[23] Khang G, Jeon EK, Rhee JM, Lee I, Lee SJ, Lee HB, Macromol. Res., 11(5), 334 (2003)

[24] Kingham PJ, Kalbermatten DF, Mahay D, Armstrong SJ, Wiberg M, Terenghi G, Exp. Neurol., 207, 267 (2007)

[25] Jang JW, Park KS, Kim SH, Tissue Eng. Regen. Med., 2, 34 (2005)

[26] Khang G, Jeon JH, Cho JC, Lee HB, Polym.(Korea), 23(3), 471 (1999)

[27] Khang G, Kim MS, Cho SH, Tissue Eng. Regen. Med., 1, 9 (2004)

[28] Sagnella S, Anderson E, Sanabria N, Marchant RE, Marchant K, Tissue Eng., 11, 226 (2005)

[29] Iwasaki Y, Sawada S, Nakabayashi N, Khang G, Lee HB, Ishihara K, Biomaterials., 20, 2185 (1999)

[30] Khang GS, Kim MS, Min BH, Lee IW, Rlee JM, Lee HB, Tissue Eng. Regen. Med., 3, 376 (2006)

[31] Morrissey TK, Leitman NK, Bunge RP, J. Neurosci., 11, 2433 (1991)

[32] Liu H, Fan H, Wang Y, Toh SL, Goh JC, Biomaterials., 29, 662 (2008)

[33] Park KS, Kim SM, Kim MS, Lee I, Rhee JM, Lee HB, Khang G, Polym.(Korea), 30(5), 445 (2006)

[34] Oh YA, Kim SH, Lee SJ, Yoo JJ, van Dyke M, Rhee JM, Khang G, Polym.(Korea), 32(5), 403 (2008)

[35] Li C, Vepari C, Jina H, Kim HJ, Kaplan DL, Biomaterials., 27, 3115 (2006)