In this experimental study, the performance of a selected diesohol-biodiesel blend consisting of 84.00% (vol.) diesel, 0.25% hydrous ethanol, 4.75% anhydrous ethanol, and 11.00% biodiesel was tested on a light-duty truck with a 2.5L inter-cooled direct injection diesel engine. Emission and engine performance tests for the diesohol-biodiesel blend and regular diesel were conducted on a chassis dynamometer using a Bangkok Driving Cycle. It was found that the two fuels show no significant difference on the emissions of CO2 and NOx. However, running the test engine on diesohol-biodiesel blend resulted in lower particulate matter and CO emissions compared to regular diesel. It also yielded a lower maximum power output due to its lower heating value. There was no significant difference in the fuel consumption of the two fuels.