Poly(L-lactide acid)-blend-gelatin (PLLA-gelatin) nanofibers were successfully fabricated by means of electrospinning. The different material components characterizing the properties of electrospun PLLA/G nanofibers were measured and the effect of PLLA weight ratios on such properties as morphologies, physical and chemical structure and mechanical profiles were analyzed. It was found that the fibers diameter increases and the ultimate tension-stress enhances with increased PLLA weight ratio. The analysis of X-ray diffractometry, differential scanning calorimetry, and Fourier-transform infrared spectra demonstrated that the resultant nanofibers from electrospinning of PLLA-gelatin solution are simple blends of these two components. (C) 2010 Wiley Periodicals, Inc. J Appl Polym Sci 117: 542-547, 2010