Solid lipid nanoparticles coated with silk fibroin

Teak Kwan Kwona; Kun Bin Lim; Jin-Chul Kim

Journal of Industrial and Engineering Chemistry, Vol.17, No.1, 10-13, January, 2011

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Solid lipid nanoparticles coated with silk fibroin

Teak Kwan Kwona, Kun Bin Lim¹, and Jin-Chul Kim^†

Division of Biotechnology & Bioengineering and Institute of Bioscience and Biotechnology, Kangwon National University, 192-1, Hyoja 2-dong,Chunchon, Kangwon-do 200-701, Republic of Korea
¹FineCo Ltd., Bio Venture Plaza, 198-60, Hupyong dong, Chunchon, Kangwon-do 200-160, Republic of Korea

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Solid lipid nanoparticles (SLNs) composed of stearic acid (SA) and ceramide (Cer) were prepared by an emulsification and solidification method using sodium lauryl sulfate (an anionic surfactant) as a stabilizer. And then, the SLN was coated with silk fibroin (SF) under an acidic condition by an electrostatic interaction. The size was tens of nanometers to hundreds of nanometers, and the coat around SA/Cer SLN was observed on the TEM photos. Following the thermograms of the SLN with and without SF coating, two phases, of which crystalline structures were different from each other, existed in the SLN. In vitro skin permeation experiment, the flux of Cer in 24 h were about 26 mg/cm2/h with SLN without SF coating, and about 55 mg/cm2/h with SLN with coating. The SF coat of nanoparticles was positively charged, so it would strongly interact with negatively charged skins, enhancing the skin permeation of Cer.

Keywords:Solid lipid nanoparticles;Stearic acid;Ceramide;In vitro permeation

[References]

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Singh AK, Das J, Biophys. Chem., 73, 155 (1998)
Kim JC, Song ME, Kim MJ, Lee EJ, Park SK, Rang MJ, Ahn HJ, Colloid Surf. B: Biointerfaces., 26, 235 (2002)
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Mei Z, Chen H, Weng T, Yang Y, Yang X, Eur. J. Pharm. Biopharm., 56, 189 (2003)
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Tabatt K, Kneuer C, Sameti M, Olbrich C, Muller RH, Lehr CM, Bakowsky U, J. Control. Release., 97, 321 (2004)
Salomone B, Ponti R, Gasco MR, Ugazio E, Quaglino P, Osella-Abate S, Bernengo MG, Clin. Exp. Metastas., 18, 663 (2001)
Pandey R, Sharma S, Khuller GK, Tuberculosis., 85, 415 (2005)
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Li Z, Lin X, Yu L, Li X, Geng F, Zheng L, J. Disper. Sci. Technol., 30, 1008 (2009)
Kawasaki N, Araki M, Nakamura T, Tanada S, J. Colloid Interface Sci., 238(1), 215 (2001)
Katrin M, Katrin K, Aarti N, Yogeshvar NK, Richard HG, Eur. J. Pharm. Biopharm., 52, 103 (2001)
Connors KA, J. Am. Chem. Soc., 97, 1325 (1997)
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Piemi MPY, Korner D, Benita S, Marty JP, J. Control. Release., 58, 177 (1999)

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[1] Barichello JM, Handa H, Kisyuku M, Shibata T, Ishida T, Kiwada H, J. Control. Release., 115, 94 (2006)

[2] Manosroi A, Kongkaneramit L, Manosroi J, Int. J. Pharm., 270, 279 (2004)

[3] Singh AK, Das J, Biophys. Chem., 73, 155 (1998)

[4] Kim JC, Song ME, Kim MJ, Lee EJ, Park SK, Rang MJ, Ahn HJ, Colloid Surf. B: Biointerfaces., 26, 235 (2002)

[5] Kim JC, Lee MH, Rang MJ, Drug Deliv., 10, 119 (2003)

[6] Patravale VB, Mandawgade SD, Int. J. Cosmet. Sci., 30, 19 (2008)

[7] Gregoriadis G, Liposomes as Drug Carriers, Wiley, New York (1988)

[8] Morel S, Terreno E, Ugazio E, Aime S, Gasco MR, Eur. J. Pharm. Biopharm., 45, 157 (1998)

[9] Mei Z, Chen H, Weng T, Yang Y, Yang X, Eur. J. Pharm. Biopharm., 56, 189 (2003)

[10] Mei Z, Li X, Wu Q, Hu S, Yang X, Pharmacol. Res., 51, 345 (2005)

[11] Tabatt K, Kneuer C, Sameti M, Olbrich C, Muller RH, Lehr CM, Bakowsky U, J. Control. Release., 97, 321 (2004)

[12] Salomone B, Ponti R, Gasco MR, Ugazio E, Quaglino P, Osella-Abate S, Bernengo MG, Clin. Exp. Metastas., 18, 663 (2001)

[13] Pandey R, Sharma S, Khuller GK, Tuberculosis., 85, 415 (2005)

[14] Altman GH, Diaz F, Jakuba C, Calabro T, Horan RL, Chen J, Lu H, Richmond J, Kaplan DL, Biomaterials., 24, 401 (2003)

[15] Li Z, Lin X, Yu L, Li X, Geng F, Zheng L, J. Disper. Sci. Technol., 30, 1008 (2009)

[16] Kawasaki N, Araki M, Nakamura T, Tanada S, J. Colloid Interface Sci., 238(1), 215 (2001)

[17] Katrin M, Katrin K, Aarti N, Yogeshvar NK, Richard HG, Eur. J. Pharm. Biopharm., 52, 103 (2001)

[18] Connors KA, J. Am. Chem. Soc., 97, 1325 (1997)

[19] Uekama K, Hirayama F, Irie T, J. Am. Chem. Soc., 98, 2045 (1998)

[20] Piemi MPY, Korner D, Benita S, Marty JP, J. Control. Release., 58, 177 (1999)