MATHEMATICAL MODELING AND OPTIMIZATION OF PLASMID-HARBORING AND CHROMOSOME-INTEGRATED RECOMBINANT YEAST CULTURE PROCESSES

Cha HJ; Yoo YJ

Korean Journal of Chemical Engineering, Vol.13, No.2, 172-180, March, 1996

Cha HJ, Yoo YJ

For the enhanced secretion of foreign proteins, the mathematical modeling for overall recombinant yeast culture considering protein secretion dynamics is important because we can understand and predict the behavior of biosynthesis and secretion of foreign protein and can develop control strategies for feeding with these model equa-tions. In this research, the mathematical modeling and simulation considering protein secretion dynamics for recombi-nant yeast cultures that contain multicopy plasmids or chromosomally integrated genes were performed for optimization of foreign glucoamylase production. The optimal feeding policy for maximizing glucoamylase production was suggested in fed-batch culture. By introducing this optimal feeding policy, final glucoamylase activity and productivity of fed-batch culture were significantly increased compared with those of batch culture in both recombinant yeasts.

Keywords:Plasmid-Harboring Recombinant Yeast;Chromosome-Integrated Recombinant Yeast;Glucoamylase;Mathematical Modeling;Optimization

[References]

Aiba S, Shoda M, Nagatani M, Biotechnol. Bioeng., 10, 845 (1968)
Bentley WE, Kompala DS, Biotechnol. Bioeng., 33, 49 (1989)
Carlson M, Botstein D, Cell, 28, 145 (1982)
Cha HJ, Yoo YJ, Ahn JH, Kang HS, Biotechnol. Lett., 14, 747 (1992)
Cha HJ, Yoo YJ, Korean J. Chem. Eng., 12(5), 567 (1995)
Cha HJ, "Biosynthesis and Secretion of Foreign Glucoamylase Using Plasmid-Harboring and Chromosome-Integrated Recombinant Saccharomyces cerevisiaes," Ph.D. Thesis, Seoul Nat. Univ., Seoul, Korea (1995)
Chu FK, Maley F, J. Biol. Chem., 255, 6392 (1980)
Hjortso MA, Bailey JE, Biotechnol. Bioeng., 26, 528 (1984)
Hong J, Biotechnol. Bioeng., 34, 563 (1988)
Kingsman SM, Kingsman AJ, Mellor J, Trends Biotechnol., 5, 53 (1987)
Lee SB, Seressiotis A, Bailey JE, Biotechnol. Bioeng., 27, 1699 (1985)
Luong JHT, Biotechnol. Bioeng., 27, 280 (1985)
Modak JM, Lim HC, Tayeb YJ, Biotechnol. Bioeng., 28, 1396 (1986)
Navak M, Strehaiano P, Moreno M, Goma G, Biotechnol. Bioeng., 23, 201 (1981)
Ollis DF, Chan HT, Biotechnol. Bioeng., 24, 2583 (1982)
Park SJ, Ramirez WF, AIChE J., 34, 1550 (1988)
Park SJ, Ramirez WF, Biotechnol. Bioeng., 33, 272 (1989)
Park TH, Seo JH, Lim HC, Biotechnol. Bioeng., 34, 1167 (1989)
Patkar A, Seo JH, Lim HC, Biotechnol. Bioeng., 41, 1066 (1993)
Seo JH, Bailey JE, Biotechnol. Bioeng., 27, 156 (1985)
Schwartz LS, Jansen NB, Ho NWY, Tsao GT, Biotechnol. Bioeng., 32, 733 (1987)
Wittrup KD, Bailey JE, Biotechnol. Bioeng., 31, 304 (1987)
Yamashita I, Suzuki K, Fukui S, J. Bacteriol., 161, 567 (1985)

[Referenced By]

[1] Aiba S, Shoda M, Nagatani M, Biotechnol. Bioeng., 10, 845 (1968)

[2] Bentley WE, Kompala DS, Biotechnol. Bioeng., 33, 49 (1989)

[3] Carlson M, Botstein D, Cell, 28, 145 (1982)

[4] Cha HJ, Yoo YJ, Ahn JH, Kang HS, Biotechnol. Lett., 14, 747 (1992)

[5] Cha HJ, Yoo YJ, Korean J. Chem. Eng., 12(5), 567 (1995)

[6] Cha HJ, "Biosynthesis and Secretion of Foreign Glucoamylase Using Plasmid-Harboring and Chromosome-Integrated Recombinant Saccharomyces cerevisiaes," Ph.D. Thesis, Seoul Nat. Univ., Seoul, Korea (1995)

[7] Chu FK, Maley F, J. Biol. Chem., 255, 6392 (1980)

[8] Hjortso MA, Bailey JE, Biotechnol. Bioeng., 26, 528 (1984)

[9] Hong J, Biotechnol. Bioeng., 34, 563 (1988)

[10] Kingsman SM, Kingsman AJ, Mellor J, Trends Biotechnol., 5, 53 (1987)

[11] Lee SB, Seressiotis A, Bailey JE, Biotechnol. Bioeng., 27, 1699 (1985)

[12] Luong JHT, Biotechnol. Bioeng., 27, 280 (1985)

[13] Modak JM, Lim HC, Tayeb YJ, Biotechnol. Bioeng., 28, 1396 (1986)

[14] Navak M, Strehaiano P, Moreno M, Goma G, Biotechnol. Bioeng., 23, 201 (1981)

[15] Ollis DF, Chan HT, Biotechnol. Bioeng., 24, 2583 (1982)

[16] Park SJ, Ramirez WF, AIChE J., 34, 1550 (1988)

[17] Park SJ, Ramirez WF, Biotechnol. Bioeng., 33, 272 (1989)

[18] Park TH, Seo JH, Lim HC, Biotechnol. Bioeng., 34, 1167 (1989)

[19] Patkar A, Seo JH, Lim HC, Biotechnol. Bioeng., 41, 1066 (1993)

[20] Seo JH, Bailey JE, Biotechnol. Bioeng., 27, 156 (1985)

[21] Schwartz LS, Jansen NB, Ho NWY, Tsao GT, Biotechnol. Bioeng., 32, 733 (1987)

[22] Wittrup KD, Bailey JE, Biotechnol. Bioeng., 31, 304 (1987)

[23] Yamashita I, Suzuki K, Fukui S, J. Bacteriol., 161, 567 (1985)