The application of colloidal nonocrystals in various devices requires their assembly into well-defined mono- or multilayers. We explore the possibilities of the Langinuir Schaelcr technique to make such layers, using CdSe quantum dots as a model system. The layer quality is assessed using atomic force microscopy, transmission electron microscopy, and UV-vis absorption spectroscopy. For hydrophobic substrates, we find that the Langmuir-Schaefer technique is an excellent tool for controlled multilayer production. With hydrophilic substrates, dewetting induces a cellular superstructure. Combination with photolithography leads to micropatterned multilayers. and combination of different nanocrystal sizes allows for the formation of 2D binary superstructures.