We have combined a topographically patterned agarose microstamp with an electrode substrate to develop a novel printing device that internally contains an electrochemical system for a controlled supply of reactive ink to the stamp surface. The 10 wt % agarose gel containing 0.1 M PBS + 25 mM K Br showed suitable elasticity for forming stamps and served as the electrolytic medium for the electrochemical oxidation of Br- to generate H BrO. The electrode substrate patched with an agarose stamp having 50-mu m-high bumps was used for the spatially confined detachment of heparin/polyethyleneimine precoated on glass substrates, followed by micropatterned adsorption of fibronectin. Using the microelectrode array, the addressable micropatterning of protein by the controlled delivery of H BrO to each bump was demonstrated.