Hollow fiber membranes (HFMs) are extensively used in different industrial applications. Under some controlled fabrication conditions, axially aligned grooves can be formed on the HFM inner surface during typical immersion precipitation-based phase inversion fabrication processes. Such grooved HFMs are found to be promising for nerve repair and regeneration. The axially aligned grooves appearing on the inner surface of the membrane are considered as hydrodynamic instability patterns. During the immersion precipitation process, a transfer of solvent takes place across the interface between a polymer solution and a nonsolvent. This solvent transfer induces gradients of interfacial tension that are considered to be the driving mechanism for Marangoni instability. The onset of the stationary instability is studied by means of a linear instability theory, and the critical and maximum wavenumbers are determined and discussed in terms of the dimensionless groups characterizing the system: viscosity ratio, diffusivity ratio. Schmidt number, crispation number, adsorption number. Marangoni number, and the polymer bulk concentration. A good agreement is found between the predicted wavelength of the most dangerous wave and the experimental groove width. Consequently, solutal Marangoni instability can explain the groove formation mechanism in HFM fabrication.