Extractive bioconversion of xylan for production of xylobiose and xylotriose using a PEG6000/sodium citrate aqueous two-phase system

Xin Li; Zhina Lian; Bo Dong; Yong Xu; Qiang Yong; Shiyuan Yu

Korean Journal of Chemical Engineering, Vol.28, No.9, 1897-1901, September, 2011

DOI10.1007/s11814-011-0063-z Export Citation

Extractive bioconversion of xylan for production of xylobiose and xylotriose using a PEG6000/sodium citrate aqueous two-phase system

Xin Li^{1, 2, †}, Zhina Lian^{1, 2}, Bo Dong^{1, 2}, Yong Xu^{1, 2}, Qiang Yong^{1, 2}, and Shiyuan Yu^{1, 2}

¹Key Laboratory of Forest Genetics & Biotechnology, Ministry of Education, Nanjing Forestry University, Nanjing 210037, China
²College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, China

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Aqueous two-phase system (ATPS) was applied for extraction bioconversion of xylan by xylanase from Trichoderma viride. Phase diagrams for poly (ethylene glycol) (PEG) and sodium citrate were determined at room temperature. The ATPS composed of 12.99% (w/w) PEG6000 and 12.09% (w/w) sodium citrate was favorable for partition of xylanase and used for extraction bioconversion of xylan. Batch hydrolysis demonstrated that higher concentrations of xylobiose and xylotriose were obtained in the PEG6000/sodium citrate ATPS compared to those in the aqueous system. These results present the potential feasibility of production of xylo-oligosaccharides by extraction bioconversion in ATPS.

Keywords:Extractive Bioconversion;Xylanase;Xylobiose;Xylotriose;Aqueous Two-phase System

[References]

Yoshinari I, Hisaya A, JP Patent, 2,001,226,409 (2001)
Okazaki M, Fujikawa S, Matsumoto N, J. Jpn. Soc. Nutr. Food Sci., 43, 395 (1990)
Suwa Y, Koga K, Fujikawa S, Okazaki M, US Patent, 5,939,309 (1999)
Yoon HH, Korean J. Chem. Eng., 15(6), 631 (1998)
Nabarlatz D, Ebringerova A, Montane D, Carbohyd. Polym., 69, 20 (2007)
Katapodis P, Kavarnou A, Kintzios S, Pistola E, Kekos D, Macris BJ, Christakopoulos P, Biotechnol. Lett., 24(17), 1413 (2002)
Vazquez MJ, Alonso JL, Dominguez H, Parajo JC, Trends Food Sci. Technol., 11, 387 (2000)
Tan SS, Li DY, Jiang ZQ, Zhu YP, Shi B, Li LT, Bioresour. Technol., 99(1), 200 (2008)
Pellerin P, Gosselin M, Lepoutre JP, Samain E, Debeire P, Enzym. Microb. Technol., 13, 617 (1991)
Yuan QP, Zhang H, Qian ZM, Yang XJ, J. Chem. Technol. Biotechnol., 79(10), 1073 (2004)
Diamond AD, Hsu JT, Adv. Biochem. Eng./Biotechnol., 47, 89 (1992)
Hatti-Kaul R, Aqueous two-phase systems: Methods and protocols, Humana Press, New Jersey (2000)
Chen X, Xu G, Li X, Li Z, Ying H, Process Biochem., 43, 765 (2008)
Jain A, Johri BN, Bioresour. Technol., 67(2), 205 (1999)
Wongmongkol N, Prichanont S, Korean J. Chem. Eng., 23(1), 71 (2006)
Kaul R, Mattiasson B, Appl. Microbiol. Biotechnol., 24, 259 (1986)
Sharma S, Teotia S, Gupta MN, Enzyme Microb. Technol., 32(2), 337 (2003)
Li M, Kin JW, Peeples TL, J. Biotechnol., 93, 15 (2002)
Canales M, Ballesteros C, Moreno-Cid JA, Espinosa AM, Villar M, de la Fuent J, Biochem. Eng. J., 46, 105 (2009)
Kaul A, Pereira RA, Asenjo JA, Merchuk JC, Biotechnol. Bioeng., 48(3), 246 (1995)
Miller GL, Anal. Chem., 31, 426 (1959)
Asenjo JA, Turner RE, Mistry SL, Kaul A, J. Chromatogr.A., 668, 129 (1994)
da Silva CAS, Coimbra JSR, Rojas EEG, Teixeira JAC, Process Biochem., 44, 1213 (2009)
Mohamadi HS, Omidinia E, J. Chromatogr. B., 854, 273 (2007)
Xu Y, He G, Li J, J. Zhejiang Univ. Sci., 6B, 1087 (2005)
Yang S, Huang Z, Jiang Z, Li L, Process Biochem., 43, 56 (2008)
Albertsson P, Partition of cell particles and macromolecules in polymer two-phase systems, Academic Press Inc., New York (1970)
Borjesson J, Engqvist M, Sipos B, Tjerneld F, Enzyme Microb. Technol., 41(1-2), 186 (2007)

[Referenced By]

[1] Yoshinari I, Hisaya A, JP Patent, 2,001,226,409 (2001)

[2] Okazaki M, Fujikawa S, Matsumoto N, J. Jpn. Soc. Nutr. Food Sci., 43, 395 (1990)

[3] Suwa Y, Koga K, Fujikawa S, Okazaki M, US Patent, 5,939,309 (1999)

[4] Yoon HH, Korean J. Chem. Eng., 15(6), 631 (1998)

[5] Nabarlatz D, Ebringerova A, Montane D, Carbohyd. Polym., 69, 20 (2007)

[6] Katapodis P, Kavarnou A, Kintzios S, Pistola E, Kekos D, Macris BJ, Christakopoulos P, Biotechnol. Lett., 24(17), 1413 (2002)

[7] Vazquez MJ, Alonso JL, Dominguez H, Parajo JC, Trends Food Sci. Technol., 11, 387 (2000)

[8] Tan SS, Li DY, Jiang ZQ, Zhu YP, Shi B, Li LT, Bioresour. Technol., 99(1), 200 (2008)

[9] Pellerin P, Gosselin M, Lepoutre JP, Samain E, Debeire P, Enzym. Microb. Technol., 13, 617 (1991)

[10] Yuan QP, Zhang H, Qian ZM, Yang XJ, J. Chem. Technol. Biotechnol., 79(10), 1073 (2004)

[11] Diamond AD, Hsu JT, Adv. Biochem. Eng./Biotechnol., 47, 89 (1992)

[12] Hatti-Kaul R, Aqueous two-phase systems: Methods and protocols, Humana Press, New Jersey (2000)

[13] Chen X, Xu G, Li X, Li Z, Ying H, Process Biochem., 43, 765 (2008)

[14] Jain A, Johri BN, Bioresour. Technol., 67(2), 205 (1999)

[15] Wongmongkol N, Prichanont S, Korean J. Chem. Eng., 23(1), 71 (2006)

[16] Kaul R, Mattiasson B, Appl. Microbiol. Biotechnol., 24, 259 (1986)

[17] Sharma S, Teotia S, Gupta MN, Enzyme Microb. Technol., 32(2), 337 (2003)

[18] Li M, Kin JW, Peeples TL, J. Biotechnol., 93, 15 (2002)

[19] Canales M, Ballesteros C, Moreno-Cid JA, Espinosa AM, Villar M, de la Fuent J, Biochem. Eng. J., 46, 105 (2009)

[20] Kaul A, Pereira RA, Asenjo JA, Merchuk JC, Biotechnol. Bioeng., 48(3), 246 (1995)

[21] Miller GL, Anal. Chem., 31, 426 (1959)

[22] Asenjo JA, Turner RE, Mistry SL, Kaul A, J. Chromatogr.A., 668, 129 (1994)

[23] da Silva CAS, Coimbra JSR, Rojas EEG, Teixeira JAC, Process Biochem., 44, 1213 (2009)

[24] Mohamadi HS, Omidinia E, J. Chromatogr. B., 854, 273 (2007)

[25] Xu Y, He G, Li J, J. Zhejiang Univ. Sci., 6B, 1087 (2005)

[26] Yang S, Huang Z, Jiang Z, Li L, Process Biochem., 43, 56 (2008)

[27] Albertsson P, Partition of cell particles and macromolecules in polymer two-phase systems, Academic Press Inc., New York (1970)

[28] Borjesson J, Engqvist M, Sipos B, Tjerneld F, Enzyme Microb. Technol., 41(1-2), 186 (2007)