Nickel oxide (NiO) thin films were prepared on glass substrates at various bias voltages using dc reactive magnetron sputtering technique. The influence of substrate bias voltage on structural, optical and electrical properties was systematically investigated using X-ray diffraction (XRD), SEM, EDS, spectrophotometer and Hall effect studies. The NiO films are crystalline with preferential growth along (2 0 0) plane. The NiO films exhibit optical transmittance of 55% and direct band gap of 3.78 eV at the substrate bias voltage of -75 V. The electrical resistivity decreases as substrate bias voltage increases from 0 to -75 V thereafter it was slightly increased. (C) 2010 Elsevier B.V. All rights reserved.