The water-miscible alcohols (ethanol, 1-propanpol, 2-propanol) were used to form aqueous two-phase systems with K2HPO4 and Na2HPO4. Meanwhile, the organic citrates were also investigated as the phase-separation salts. The effects of the types and concentrations of phase-forming substances as well as temperature on the formation of aqueous two-phase systems were discussed. The alcohol (ethanol, 1-propanpol, 2-propanol)-salt (K2HPO4, K3C6H5O7, (NH4)3C6H5O7) aqueous two-phase systems were used to extract Rhodamine B and methyl orange in aqueous solution. It proved that the water-miscible alcohol-salt (inorganic salt, organic salt) aqueous two-phase systems can be both used to extract hydrophilic targets directly without adding any reaction reagents. The aqueous two-phase extraction process was also discussed.