Rice straw is the main grain straw and is produced in large quantities every year in Korea. Pretreatment of lignocellulosic biomass using soaking process was carried out mild conditions at atmospheric pressure and temperature of 60 ℃. We found enzymatic hydrolysis condition of pretreated biomass. In case of a rice straw, compared with previous lignocellulosic biomass, we found that hydrolysis time was a shorter than others. Hydrolysis of SAA-treated rice straw has shown conversion rate was higher at 50 ℃. Hydrolysis was ended between 40~48 hour. Glucose conversion rate was higher when enzyme loading is 65 FPU/ml and 32 CbU/ml. When substrate concentration was 5%(w/v), it was that conversion rate was 83.8% after hydrolysis for 72 hr. In simultaneous saccharification and fermentation(SSF) experiment about SAA-treated rice straw, ethanol productive yield was highest from 40 ℃. The yield of that time was 33.05% from 48 hour.