Self-Crosslinked Polyethylenimine Nanogels for Enhanced Intracellular Delivery of siRNA

Chunsoo Kim; Yuhan Lee; Soo Hyeon Lee; Jee Seon Kim; Ji Hoon Jeong; Tae Gwan Park

Macromolecular Research, Vol.19, No.2, 166-171, February, 2011

DOI10.1007/s13233-011-0207-3 Export Citation

Self-Crosslinked Polyethylenimine Nanogels for Enhanced Intracellular Delivery of siRNA

Chunsoo Kim, Yuhan Lee, Soo Hyeon Lee, Jee Seon Kim, Ji Hoon Jeong¹, and Tae Gwan Park^†

Department of Biological Sciences and Graduate School of Nanoscience and Technology, Korea Advanced Institute of Science and Technology, Deajeon, 305-701, Korea
¹School of Pharmacy, Sungkyunkwan University, Suwon, 440-746, Korea

E-mail:

Branched polyethylenimine (bPEI) (Mw=25 k) conjugated with catechol groups (PEI-C) was crosslinked to produce PEI-catechol nanogels (PCNs), which were used as a new class of small interfering RNA (siRNA) delivery carriers. The conjugated catechol groups were crosslinked with the primary amines of PEI under basic conditions. The size and morphology of the PCNs were analyzed by dynamic light scattering (DLS) and atomic force microscopy (AFM). The resulting PCNs were quite stable and had an average diameter of 111.4 ± 14.8 nm in an aqueous solution. Compared to bPEI, the PCNs exhibited much lower cytotoxicity, and formed more stable complexes with siRNA. The PCNs/siRNA complexes exhibited enhanced cellular uptake and promoted gene silencing efficiency, suggesting that they can be used potentially as a less cytotoxic siRNA carrier.

Keywords:siRNA;polyethylenimine;nanogel;catechol;bio-inspired.

[References]

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Lee H, Dellatore SM, Miller WM, Messersmith PB, Science, 317, 426 (2007)
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[Referenced By]

[1] Carthew RW, Sontheimer EJ, Cell, 136, 642 (2009)

[2] Oh YK, Park TG, Adv. Drug Deliv. Rev., 61, 850 (2009)

[3] Howard KA, Adv. Drug Deliv. Rev., 61, 710 (2009)

[4] Elbakry A, Zaky A, Liebl R, Rachel R, Goepferich A, Breunig M, Nano Lett., 9, 2059 (2009)

[5] Kim J, Lee SH, Choe J, Park TG, J. Gene. Med., 11, 804 (2009)

[6] Woodrow KA, Cu Y, Booth CJ, Saucier-Sawyer JK, Wood MJ, Saltzman WM, Nat. Mater., 8(6), 526 (2009)

[7] Lee JH, Lee K, Moon SH, Lee Y, Park TG, Cheon J, Angew. Chem.-Int. Edit., 48, 4174 (2009)

[8] Lee SH, Bae KH, Kim SH, Lee KR, Park TG, Int. J. Pharm., 364, 94 (2008)

[9] Jeong JH, Mok H, Oh YK, Park TG, Bioconjugate Chem., 20, 5 (2009)

[10] Kim SH, Jeong JH, Lee SH, Kim SW, Park TG, J. Control. Release, 129, 107 (2008)

[11] Kim WJ, Kim SW, Pharm. Res., 26, 657 (2009)

[12] Mok H, Park TG, Macromol. Biosci., 9, 731 (2009)

[13] Akinc A, Thomas M, Klibanov AM, Langer R, J. Gene. Med., 7, 657 (2005)

[14] Boussif O, Lezoualc'h F, Zanta MA, Mergny MD, Scherman D, Demeneix B, Behr JP, Proc. Nat'l. Acad. Sci. USA, 92, 7297 (1995)

[15] Neu M, Fischer D, Kissel T, J. Gene. Med., 7, 992 (2005)

[16] Elouahabi A, Ruysschaert JM, Mol. Ther., 11, 336 (2005)

[17] Pozharski EV, MacDonald RC, Mol. Pharm., 4, 962 (2007)

[18] Clamme JP, Azoulay J, Mely Y, Biophys. J., 84, 1960 (2003)

[19] Putnam D, Gentry CA, Pack DW, Langer R, PNAS, 98, 1200 (2001)

[20] Burzio LA, Waite JH, Biochemistry, 39, 11147 (2000)

[21] Waite JH, Qin X, Biochemistry, 40, 2887 (2001)

[22] Lee H, Scherer NF, Messersmith PB, PNAS, 103, 12999 (2006)

[23] Waite JH, Andersen NH, Jewhurst S, Sun CJ, J. Adhes., 81(3-4), 297 (2005)

[24] Yu ME, Hwang JY, Deming TJ, J. Am. Chem. Soc., 121(24), 5825 (1999)

[25] Lee H, Lee Y, Statz AR, Rho J, Park TG, Messersmith PB, Adv. Mater., 20(9), 1619 (2008)

[26] Sever MJ, Weisser JT, Monahan J, Srinivasan S, Wilker JJ, Angew. Chem.-Int. Edit., 43, 448 (2004)

[27] Xu CJ, Xu KM, Gu HW, Zheng RK, Liu H, Zhang XX, Guo ZH, Xu B, J. Am. Chem. Soc., 126(32), 9938 (2004)

[28] Kang SM, Rho J, Choi IS, Messersmith PB, Lee H, J. Am. Chem. Soc., 131(37), 13224 (2009)

[29] Lee H, Lee BP, Messersmith PB, Nature, 448, 338 (2007)

[30] Lee H, Dellatore SM, Miller WM, Messersmith PB, Science, 317, 426 (2007)

[31] Podsiadlo P, Liu ZQ, Paterson D, Messersmith PB, Kotov NA, Adv. Mater., 19(7), 949 (2007)

[32] Lee YH, Lee H, Kim YB, Kim JY, Hyeon T, Park H, Messersmith PB, Park TG, Adv. Mater., 20(21), 4154 (2008)

[33] Mok H, Park JW, Park TG, Bioconjugate Chem., 18, 1483 (2007)

[34] Aravindan L, Bicknell KA, Brooks G, Khutoryanskiy VV, Williams AC, Int. J. Pharm., 378, 201 (2009)

[35] Fischer D, Li Y, Ahlemeyer B, Krieglstein J, Kissel T, Biomaterials, 24, 1121 (2003)

[36] Lee H, Mok H, Lee S, Oh YK, Park TG, J. Control. Release, 119, 245 (2007)

[37] Mok H, Park TG, Biopolymers, 89, 881 (2008)