Preparation of Sericin Microparticles by Electrohydrodynamic Spraying and Their Application in Drug Delivery

Hanjin Oh; Moo Kon Kim; Ki Hoon Lee

Macromolecular Research, Vol.19, No.3, 266-272, March, 2011

DOI10.1007/s13233-011-0301-6 Export Citation

Preparation of Sericin Microparticles by Electrohydrodynamic Spraying and Their Application in Drug Delivery

Hanjin Oh, Moo Kon Kim, and Ki Hoon Lee^†

Department of Biosystems & Biomaterials Science and Engineering, Seoul National University, Seoul, 151-921, Korea

E-mail:

Sericin (SS) is a protein present in silkworm cocoons that is normally discarded in the degumming process. Recycling sericin can expand the library of natural polymers used in the materials field. In this study, sericin microparticles were prepared using an electrohydrodynamic (EHD) spraying technique. The sizes of the SS microparticles, which can be controlled by the concentration of the dope solution, flow rate and applied voltage, were in the range of 100-300 μm. The swelling and dissolution of SS microparticles were suppressed at pH 2.2 and increased at pH 7.4, indicating their potential in oral drug delivery applications. Diclofenac was used as a model drug, and its release from SS microparticles was suppressed in the stomach-simulated fluid but was increased in the small intestine-simulated fluid.

Keywords:silk;sericin;microparticle;electrohydrodynamic spraying;durg delivery.

[References]

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Takasu Y, Yamada H, Tsubouchi K, J. Insect Biotechnol. Sericol., 71, 151 (2002)
Aramwit P, Kanokpanont S, Nakpheng T, Srichana T, Int. J. Mol. Sci., 11(5), 2200 (2010)
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Sano M, Tamada Y, Niwa K, Morita T, Yoshino G, J. Biomater. Sci.-Polym. Ed., 20, 773 (2009)
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Jaworek A, Krupa A, Exp. Fluids, 27, 43 (1999)
Oh H, Lee JY, Kim AR, Ki CS, Kim JW, Park YH, Lee KH, Fiber. Polym., 8, 470 (2007)
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Wu YQ, MacKay JA, McDaniel JR, Chilkoti A, Clark RL, Biomacromolecules, 10(1), 19 (2009)
Valo H, Peltonen L, Vehvilainen S, Karjalainen M, Kostiainen R, Laarksonen T, Hirvonen J, Small, 5, 1791 (2009)
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[Referenced By]

[1] Takasu Y, Yamada H, Tsubouchi K, Biosci. Biotechnol. Biochem., 66, 2715 (2002)

[2] Takasu Y, Yamada H, Tsubouchi K, J. Insect Biotechnol. Sericol., 71, 151 (2002)

[3] Aramwit P, Kanokpanont S, Nakpheng T, Srichana T, Int. J. Mol. Sci., 11(5), 2200 (2010)

[4] Aramwit P, Damrongsakkul S, Kanokpanont S, Srichana T, Biotechnol. Appl. Biochem., 55, 91 (2010)

[5] Wu W, Li WJ, Wang LQ, Tu KH, Sun WL, Polym. Int., 55, 513 (2006)

[6] Gimenes ML, Liu L, Feng XS, J. Membr. Sci., 295(1-2), 71 (2007)

[7] Panilaitis B, Altman GH, Chen J, Jin HJ, Karageorgiou V, Kaplan DL, Biomaterials, 24, 3079 (2003)

[8] Dewair M, Baur X, Ziegler K, J. Allergy Clin. Immunol., 76, 537 (1985)

[9] Zhang YQ, Tao ML, Shen WD, Mao JP, Chen YH, J. Chem. Technol. Biotechnol., 81(2), 136 (2006)

[10] Suzuki N, Fujimura A, Nagai T, Mizumoto I, Itami T, Hatate H, Nozawa T, Kato N, Nomoto T, Yoda B, Biofactors, 21, 329 (2004)

[11] Zhaorigetu S, Masahiro S, Watanabe H, Kato N, Biosci. Biotechnol. Biochem., 65, 2181 (2001)

[12] Sano M, Tamada Y, Niwa K, Morita T, Yoshino G, J. Biomater. Sci.-Polym. Ed., 20, 773 (2009)

[13] Nagai N, Murao T, Ito Y, Okamoto N, Sasaki M, Biol. Pharm. Bull., 32, 1594 (2009)

[14] Zhang YQ, Tao ML, Shen WD, Zhou YZ, Ding Y, Ma Y, Zhou WL, Biomaterials, 25, 3751 (2004)

[15] Zhang YQ, Ma Y, Xia YY, Shen WD, Mao JP, Xue RY, J. Control. Release, 115, 307 (2006)

[16] Wu W, Li WJ, Wang LQ, Tu KH, Sun WL, Polym. Int., 55, 513 (2006)

[17] Mandal BB, Kundu SC, Nanotechnology, 20, 1 (2009)

[18] Cloupeau M, Prunet-Foch B, J. Electrostat., 25, 165 (1990)

[19] Jaworek A, Krupa A, Exp. Fluids, 27, 43 (1999)

[20] Oh H, Lee JY, Kim AR, Ki CS, Kim JW, Park YH, Lee KH, Fiber. Polym., 8, 470 (2007)

[21] Ahn SH, Lee H, Park GM, Kim GH, Macromol. Res., 18(5), 429 (2010)

[22] Wu YQ, MacKay JA, McDaniel JR, Chilkoti A, Clark RL, Biomacromolecules, 10(1), 19 (2009)

[23] Valo H, Peltonen L, Vehvilainen S, Karjalainen M, Kostiainen R, Laarksonen T, Hirvonen J, Small, 5, 1791 (2009)

[24] Rohner TC, Lion N, Girault HH, Phys. Chem. Chem. Phys., 6, 3056 (2004)

[25] Naqwi AA, Hartman RPA, Marijnissen JCM, Part. Part. Syst. Charact., 13(2), 143 (1996)

[26] Hartman RPA, Brunner DJ, Camelot DMA, Marijnissen JCM, Scarlett B, J. Aerosol Sci., 30(7), 823 (1999)

[27] Butler JN, J. Electroanal. Chem., 14, 89 (1967)

[28] Mosher HH, Am. Dyes. Rept’r., 21, 341 (1932)