Enhanced Cellular Responses of Vascular Endothelial Cells on Poly-γ-Glutamic Acid/PU Composite Film Treated with Microwave-Induced Plasma at Atmospheric Pressure

Bong Joo Park; Byoung Ju Kwon; Jae Kyeong Kang; Mi Hee Lee; Inho Han; Jeong Koo Kim; Jong-Chul Park

Macromolecular Research, Vol.19, No.6, 537-541, June, 2011

DOI10.1007/s13233-011-0605-6 Export Citation

Enhanced Cellular Responses of Vascular Endothelial Cells on Poly-γ-Glutamic Acid/PU Composite Film Treated with Microwave-Induced Plasma at Atmospheric Pressure

Bong Joo Park^{1, 2}, Byoung Ju Kwon³, Jae Kyeong Kang³, Mi Hee Lee³, Inho Han⁴, Jeong Koo Kim^{1, 2}, and Jong-Chul Park^{3, †}

¹BK21 Bio-Ogran Tissue Regeneration Project Team, Inje University, Gimhae, 621-749, Korea
²Department of Biomedical Engineering, College of Biomedical Science and Engineering, Inje University, Gimhae, 621-749, Korea
³Brain Korea 21 Project for Medical Science, Yonsei University College of Medicine, Seoul, 120-752, Korea
⁴Department of Medical Engineering, Yonsei University College of Medicine, Seoul, 120-752, Korea

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Poly-γ-glutamic acid (γ-PGA), which is produced by microbial fermentation, is a biodegradable, hydrophilic and non-toxic biomaterial. γ-PGA has many carboxyl groups that makes it a polyanionic biopolymer with swelling ability, biocompatibility and anticoagulant activity. On the other hand, few studies have examined the effect of γ-PGA on the cellular activity of human umbilical vein endothelial cells (HUVECs). The present study evaluated the effects of γ-PGA and fabricated γ-PGA/PU composite films on the attachment and proliferation of HUVECs after treatment with microwave plasma at atmospheric pressure. The results confirmed that γ-PGA is capable of increasing the proliferation and differentiation of HUVECs to form capillary tubes with enhanced alignment and organization. Moreover, the microwave plasma modified γ-PGA/PU composite film was more hydrophilic and the surface roughness was enhanced. In addition, the attachment and proliferation of the HUVECs were increased by the plasma treatment. These results suggest that γ-PGA and surface modified γ-PGA/PU composite can be applied as bioactive and biocompatible materials in vascular tissue engineering but further testing of γ-PGA and γ-PGA/PU composite films will be needed to confirm the potential effects for use in vascular applications.

Keywords:poly-γ-glutamic acid;polyurethane;human umbilical vein endothelial cells;microwave-induced argon plasma.

[References]

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Seifalian AM, Tiwari A, Hamilton G, Salacinski HJ, Artif. Organs, 26, 307 (2002)
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Williamson MR, Black R, Kielty C, Biomaterials, 27, 3608 (2006)
Lelah MD, Cooper SL, Polyurethanes in Medicine, CRC Press, Boca Raton, FL, 1986.
Nichols NK, Gospodarwicz D, Kessler TR, Oslen DB, Trans. Am. Soc. Artif. Internal Organs, 27, 208 (1981)
Patterson RG, Messier A, Valentini RF, ASAIO J., 41, 625 (1995)
Hsu S, Chen WC, Biomaterials, 21, 359 (2000)
Ito Y, Imanishi Y, Crit. Rev. Biocompat., 5, 45 (1989)
Nakao A, Kaibara M, Iwaki M, Suzuki Y, Kusakabe M, Surf. Interface Anal., 24, 252 (1996)
Pignataro B, Conte E, Scandurra A, Marletta G, Biomaterials, 18, 1461 (1997)
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Ozeki E, Matsuda T, ASAIO Trans., 36, 294 (1990)
Lin H, Garcia-Echeverria C, Asakura S, Sun W, Mosher DF, Cooper SL, Biomaterials, 13, 905 (1992)
Choi WS, Bae JW, Joung YK, Park KD, Lee MH, Park JC, Kwon IK, Macromol. Res., 17(7), 458 (2009)
Otani Y, Tabata Y, Ikada Y, J. Biomed. Mater. Res., 31, 158 (1996)
USPTO Patent Application 0186804 (2009).
Lim HR, Baek HS, Lee MH, Woo YI, Han DW, Han MH, Baik HK, Choi WS, Park KD, Chung KH, Park JC, Surf. Coat. Technol., 202, 5768 (2008)
Park BJ, Seo HJ, Kim JS, Kim HL, Kim JK, Choi JB, Han IH, Hyun SO, Chung KH, Park JC, Surf. Coat. Technol., 205, S222 (2010)
Park BJ, Lee DH, Park JC, Lee IS, Lee KY, Hyun SO, Chun MS, Chung KH, Phys. Plasmas, 10, 4539 (2003)
Arnaoutova I, George J, Kleinman HK, Benton G, Angiogenesis, 12, 267 (2009)
Shen H, Hu X, Yang F, Bei J, Wang S, Biomaterials, 28, 4219 (2007)

[Referenced By]

[1] Tiwari A, Salacinski HJ, Hamilton G, Sefalian AM, Eur. J. Vasc. Surg., 21, 193 (2001)

[2] Seifalian AM, Tiwari A, Hamilton G, Salacinski HJ, Artif. Organs, 26, 307 (2002)

[3] Kumar TRS, Krishnan LK, Biomaterials, 22, 2769 (2001)

[4] Rothlein R, Dustin ML, Marlin SD, Springer TA, J. Immunol. Methods, 137, 1270 (1986)

[5] Prober JS, Gimbrone MA, Lapierre LA, Mendrick DL, Fiers W, Rothlein R, Springer TA, J. Immunol. Methods, 137, 1893 (1986)

[6] Feugier P, Black RA, Hunt JA, How TV, Biomaterials, 26, 1457 (2005)

[7] Xu C, Yang CF, Wang S, Ramakrishna S, J. Biomed. Mater. Res., 74, 154 (2004)

[8] Zhang Z, Wang Z, Liu S, Kodama M, Biomaterials, 25, 177 (2004)

[9] Andrews KD, Hunt JA, Black RA, Biomaterials, 28, 1014 (2007)

[10] Williamson MR, Black R, Kielty C, Biomaterials, 27, 3608 (2006)

[11] Lelah MD, Cooper SL, Polyurethanes in Medicine, CRC Press, Boca Raton, FL, 1986.

[12] Nichols NK, Gospodarwicz D, Kessler TR, Oslen DB, Trans. Am. Soc. Artif. Internal Organs, 27, 208 (1981)

[13] Patterson RG, Messier A, Valentini RF, ASAIO J., 41, 625 (1995)

[14] Hsu S, Chen WC, Biomaterials, 21, 359 (2000)

[15] Ito Y, Imanishi Y, Crit. Rev. Biocompat., 5, 45 (1989)

[16] Nakao A, Kaibara M, Iwaki M, Suzuki Y, Kusakabe M, Surf. Interface Anal., 24, 252 (1996)

[17] Pignataro B, Conte E, Scandurra A, Marletta G, Biomaterials, 18, 1461 (1997)

[18] Dejun L, Zhao J, Appl. Surf. Sci., 78, 195 (1994)

[19] Ozeki E, Matsuda T, ASAIO Trans., 36, 294 (1990)

[20] Lin H, Garcia-Echeverria C, Asakura S, Sun W, Mosher DF, Cooper SL, Biomaterials, 13, 905 (1992)

[21] Choi WS, Bae JW, Joung YK, Park KD, Lee MH, Park JC, Kwon IK, Macromol. Res., 17(7), 458 (2009)

[22] Otani Y, Tabata Y, Ikada Y, J. Biomed. Mater. Res., 31, 158 (1996)

[23] USPTO Patent Application 0186804 (2009).

[24] Lim HR, Baek HS, Lee MH, Woo YI, Han DW, Han MH, Baik HK, Choi WS, Park KD, Chung KH, Park JC, Surf. Coat. Technol., 202, 5768 (2008)

[25] Park BJ, Seo HJ, Kim JS, Kim HL, Kim JK, Choi JB, Han IH, Hyun SO, Chung KH, Park JC, Surf. Coat. Technol., 205, S222 (2010)

[26] Park BJ, Lee DH, Park JC, Lee IS, Lee KY, Hyun SO, Chun MS, Chung KH, Phys. Plasmas, 10, 4539 (2003)

[27] Arnaoutova I, George J, Kleinman HK, Benton G, Angiogenesis, 12, 267 (2009)

[28] Shen H, Hu X, Yang F, Bei J, Wang S, Biomaterials, 28, 4219 (2007)