Pre-Wetting Process with Helium Atmospheric Pressure Glow Discharge for Three-Dimensional Porous Scaffold

Inho Han; Barbora Vagaska; Dae Hyung Lee; Bong Joo Park; Seung-Woo Shin; Jeong Koo Kim; Seung Jin Lee; Jong-Chul Park

Macromolecular Research, Vol.19, No.7, 711-715, July, 2011

DOI10.1007/s13233-011-0701-7 Export Citation

Pre-Wetting Process with Helium Atmospheric Pressure Glow Discharge for Three-Dimensional Porous Scaffold

Inho Han¹, Barbora Vagaska^{1, 2}, Dae Hyung Lee¹, Bong Joo Park^{1, 3}, Seung-Woo Shin³, Jeong Koo Kim³, Seung Jin Lee⁴, and Jong-Chul Park^{1, 2, †}

¹Cellbiocontrol Laboratory, Department of Medical Engineering, Yonsei University College of Medicine, Seoul, 120-752, Korea
²Brain Korea 21 Project for Medical Science, Yonsei University College of Medicine, Seoul, 120-752, Korea
³Department of Biomedical Engineering, College of Biomedical Science and Engineering, Inje University, Gyeongnam, 621-749, Korea
⁴Department of Industrial Pharmacy, College of Pharmacy, Ewha Womans University, Seoul, 120-750, Korea

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Recently, three-dimensional polymer scaffolds were developed for tissue regeneration. Conventional prewetting processes require a lengthy processing time and have residue problems. In this study, a helium atmospheric pressure glow discharge (He-APGD) was employed as a pre-wetting process for porous poly-(L-lactide-co-ε-caprolactone) (PLCL) scaffold. The three minutes treated PLCL scaffold absorbed the culture media and contained grafted hydroxyl, carbonyl, and carboxyl groups. Moreover, it did not show cytotoxicity, did not require residual rinsing steps and showed a homogeneous cell distribution. The He-APGD treatment has a short processing time and promotes a homogenous cell distribution. Therefore, this pre-wetting process is expected to find many applications in the future.

Keywords:biodegradable polymer scaffold;porous polymer scaffold;plasma;surface modification.

[References]

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Xie J, Han Z, Naito M, Maeyama A, Kim SH, Kim YH, Matsuda T, J. Biomed. Mater. Res., 94B, 80 (2010)
Park CH, Hong YJ, Park K, Han DK, Macromol. Res., 18(5), 526 (2010)
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Yang SJ, Yin H, Plasma Chem. Plasma Process., 27(1), 23 (2007)
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Liu X, Tang S, Choi HK, Choi HS, Macromol. Res., 18(5), 413 (2010)
Lee WH, Kim JY, Ko YK, Reucroft PJ, Zondlo JW, Appl. Surf. Sci., 141, 107 (1999)
Gai PL, Billinge BHM, Brown AM, Carbon, 27, 41 (1989)
Kormunda M, Pavlik J, Mater. Sci. Eng., 12, 012007 (2010)
Lausmaa J, Kasemo B, Mattson H, Appl. Surf. Sci., 44, 133 (1990)
Fritz L, Hofmann D, Polymer, 39, 2561 (1998)
Chuang WY, Young TH, Wang DM, Luo RL, Sun YM, Polymer, 41(23), 8339 (2000)
Wang Y, Yang J, Yang J, Wang S, Biomaterials, 24, 3757 (2003)

[Referenced By]

[1] Kanczler JM, Oreffo ROC, Eur. Cell. Mater., 15, 110 (2008)

[2] Xie J, Han Z, Naito M, Maeyama A, Kim SH, Kim YH, Matsuda T, J. Biomed. Mater. Res., 94B, 80 (2010)

[3] Park CH, Hong YJ, Park K, Han DK, Macromol. Res., 18(5), 526 (2010)

[4] Woo YI, Lee MH, Kim HL, Park JC, Han DW, Kim JK, Tsubaki K, Chung KH, Hyun SO, Yang YI, Macromol. Res., 18(1), 90 (2010)

[5] Shin YM, Shin H, Lim YM, Macromol. Res., 18(5), 472 (2010)

[6] Meneghello G, Parker DJ, Ainsworth BJ, Perera SP, Chaudhuri JB, Ellis MJ, De Bank PA, J. Membr. Sci., 344(1-2), 55 (2009)

[7] Shearer H, Ellis MJ, Perera SP, Chaudhuri JB, Tissue Eng., 12, 2717 (2006)

[8] Mikos AG, Lyman MD, Freed LE, Langer R, Biomaterials, 15, 55 (1994)

[9] Yildirim ED, Ayan H, Vasilets VN, Fridman A, Guceri S, Sun W, Plasmas Polym., 5, 58 (2008)

[10] Raizer YP, in Gas discharge physics, Raizer YP, Ed., Springer-Verlag, Berlin, 1991, pp 52-75, 128-138.

[11] Anand V, Gowravaram MR, IEEE Trans. Plasma Sci., 37, 1811 (2009)

[12] Yang SJ, Yin H, Plasma Chem. Plasma Process., 27(1), 23 (2007)

[13] Tendero C, Tixier C, Tristant P, Desmaison J, Leprince P, Spectrochim. Acta, 61, 2 (2006)

[14] Liu X, Tang S, Choi HK, Choi HS, Macromol. Res., 18(5), 413 (2010)

[15] Lee WH, Kim JY, Ko YK, Reucroft PJ, Zondlo JW, Appl. Surf. Sci., 141, 107 (1999)

[16] Gai PL, Billinge BHM, Brown AM, Carbon, 27, 41 (1989)

[17] Kormunda M, Pavlik J, Mater. Sci. Eng., 12, 012007 (2010)

[18] Lausmaa J, Kasemo B, Mattson H, Appl. Surf. Sci., 44, 133 (1990)

[19] Fritz L, Hofmann D, Polymer, 39, 2561 (1998)

[20] Chuang WY, Young TH, Wang DM, Luo RL, Sun YM, Polymer, 41(23), 8339 (2000)

[21] Wang Y, Yang J, Yang J, Wang S, Biomaterials, 24, 3757 (2003)